中国烟草学报2025，Vol.31Issue(5)：71-82,12.DOI:10.16472/j.chinatobacco.2024.T0389
烟草NtTHF1参与调控植株生长发育与叶片衰老
The role of tobacco NtTHF1 in regulating plant growth and leaf senescence
摘要
Abstract
[Background and Objective]The plant chloroplast-associated protein gene,THF1,not only plays a key role in plant immunity but also has a significant impact on plant growth,development,and sugar signal transduction.This study aimed to identify the function of the tobacco chloroplast-associated protein NtTHF1 in the growth,development,and leaf senescence of tobacco.[Methods]The chloroplast-associated protein gene NtTHF1 was cloned from the cultivated tobacco Nicotiana tabacum'Honghua Dajinyuan'.Bioinformatic methods were used to analyze the structure and evolutionary relationships of the NtTHF1 protein.Gene editing technology was employed to create NtTHF1 gene-edited thf1 plants.Pigment content and the expression levels of pigment-related and growth-related genes were measured during the seedling and mature stages.Subcellular localization of NtTHF1 was analyzed in tobacco protoplasts.[Results]The full-length coding sequence(CDS)of the tobacco NtTHF1 gene is 969 bp,encoding a THF1 protein of 322 amino acids.The plant height and leaf area of the gene-edited thf1 plants were lower than those of the wild-type(WT)control,while the pigment content in the leaves at both the seedling and mature stages was higher than that of the control.[Conclusion]The tobacco NtTHF1 gene may participate in plant growth,development,and leaf senescence by regulating genes related to growth and pigment.The findings of this research provide data to support a deeper understanding of the potential of NtTHF1 in the genetic improvement of tobacco.关键词
烟草/NtTHF1/生长发育/叶片衰老Key words
Nicotiana tabacum/NtTHF1/plant growth and development/leaf senescence引用本文复制引用
许力,米其利,许永,向海英,刘德水,李晶,高茜,曾婉俐,蒋佳芮,王昆淼..烟草NtTHF1参与调控植株生长发育与叶片衰老[J].中国烟草学报,2025,31(5):71-82,12.基金项目
中国烟草总公司科技项目"多酚类物质代谢相关新品系配方适用性评价及应用"(110202201015(JY-15)) （110202201015(JY-15）
云南大观实验室课题、云南中烟工业有限责任公司科技项目"低芸香苷新品系的品质保障技术优化及烟叶配伍性研究"(YNDG202301YZ01、2023YJ02) （YNDG202301YZ01、2023YJ02）
