中国中药杂志2025，Vol.50Issue(18)：5016-5025,10.DOI:10.19540/j.cnki.cjcmm.20250506.401
基于肠道菌群研究沙苑子总黄酮治疗慢性肝损伤的作用机制
Mechanism of total flavonoids from Astragali Complanati Semen in treating chronic liver injury based on intestinal flora
摘要
Abstract
Based on the intestinal flora perspective,this study aims to explore the therapeutic mechanism of total flavonoids from Astragali Complanati Semen(TFACS)on chronic liver injury(CLI).Mouse CLI models were constructed by intraperitoneal injection of carbon tetrachloride.The mice were randomly divided into four groups as follows:a blank group,a model group,a silybin group,and TFACS(50 or 100 mg·kg-1)groups.The corresponding drugs were administered to each group for 6 consecutive weeks.Sixteen hours after the final administration,serum and cecal contents were collected in each group.Serum liver function indexes were determined by the automatic biochemical analyzer.The liver lesions and collagen deposition were observed by hematoxylin-eosin and Masson staining.The intestinal flora components were analyzed by 16S rDNA sequencing technology.The short chain fatty acids(SCFAs)content was analyzed by GC-MS.The cecum tissue was observed by fluorescent staining.The results show that,compared with the model group,Bacteroidetes was decreased significantly at the phylum level under the TFACS intervention.Under the TFACS intervention,Firmicutes and Proteobacteria were increased significantly.At the genus level,TFACS promotes the proliferation of Lactobacillus.At the species level,TFACS significantly increases the abundance of Roseburia faecis,while decreasing the Alistipes onderdonkii and Clostridium leptum level.In addition,TFACS intervention significantly increases the contents of SCFAs,including propionic acid,acetic acid,isovaleric acid,isobutyric acid,caproic acid,and valeric acid in intestinal contents of CLI mice,while up-regulating the expression of key structural proteins of intestinal tight junctions[occludin,and zonula occludens-1(ZO-1)].In addition,TFACS intervention effectively promotes the adhesion complex formation between intestinal epithelial cells,thereby improving intestinal barrier function.In conclusion,TFACS can improve intestinal flora composition,SCFAs formation,and intestinal barrier integrity in CLI mice,providing a research basis for TFACS' anti-CLI mechanism.关键词
沙苑子总黄酮/慢性肝损伤/肠道菌群/短链脂肪酸Key words
total flavonoids from Astragali Complanati Semen/chronic liver injury/intestinal flora/short-chain fatty acids引用本文复制引用
王洋,周瑞娜,雷莉妍,岳正刚,陈琳..基于肠道菌群研究沙苑子总黄酮治疗慢性肝损伤的作用机制[J].中国中药杂志,2025,50(18):5016-5025,10.基金项目
陕西省科技厅重点研发计划项目(2024SF-YBXM-458) （2024SF-YBXM-458）
陕西省教育厅重点科学研究计划项目(22JY019) （22JY019）
陕西高校青年创新团队建设项目(2023) （2023）
秦创原中医药产业创新聚集区项目(L2024-QCY-ZYYJJQ-X201) （L2024-QCY-ZYYJJQ-X201）
陕西省重点研发计划-重点产业创新链(群)(社会发展领域)项目(2023-ZDLSF-58) （群）
