中国中药杂志2025，Vol.50Issue(18)：5026-5035,10.DOI:10.19540/j.cnki.cjcmm.20250513.401
基于网络药理学与实验验证研究仁青芒觉通过表观遗传调控轴PRMT1/H4R3me2a/PAI-1/MCP-1缓解肝纤维化的作用和机制
Role and mechanism of Renqing Mangjue in attenuating hepatic fibrosis via PRMT1/H4R3me2a/PAI-1/MCP-1 epigenetic regulatory axis based on network pharmacology and experimental validation
摘要
Abstract
The current study aims to elucidate the underlying pharmacological mechanisms of the Tibetan medicine Renqing Mangjue against hepatic fibrosis using a research strategy integrating network pharmacology and experimental verification.Through the combination of a rat hepatic fibrosis model induced by diethylnitrosamine(DEN)and the analysis of clinical transcriptome data from the GEO database,the characteristics of its pharmacological effects were objectively evaluated,and its mechanism of action was systematically analyzed.The experimental results showed that the intervention of Renqing Mangjue significantly reduced the levels of serum hepatic fibrosis markers,including procollagen type Ⅲ(PC Ⅲ),collagen type Ⅳ(Ⅳ-C),and laminin(LN).Specifically,the medium-dose group showed a decrease of 37.8％,47.1％,and 36.6％respectively compared with the model group(all P＜0.05)and a reduced collagen deposition in the liver tissue.The mechanism study found that it down-regulated the expressions of protein arginine methyltransferase 1(PRMT1),histone H4 arginine 3 dimethylation(H4R3me2a),plasminogen activator inhibitor-1(PAI-1),and monocyte chemoattractant protein 1(MCP-1)and inhibited the PRMT1/H4R3me2a/PAI-1/MCP-1 signaling axis,thereby inhibiting the activation of hepatic stellate cells and the excessive deposition of the extracellular matrix.This study is the first to clarify that Renqing Mangjue may exert an anti-hepatic fibrosis effect through the epigenetic regulation pathway,providing an experimental basis for its clinical application and offering a new paradigm for modern research on Tibetan medicine compounds.关键词
仁青芒觉/肝纤维化/细胞外基质沉积/PRMT1/生物分子网络Key words
Renqing Mangjue/hepatic fibrosis/extracellular matrix deposition/PRMT1/biomolecular network引用本文复制引用
肖自青,刘颖,张彦琼,林娜,黄丰,杨竹雅,马兆臣,邹洁,胥明珠,陈沛萍,张楚,杨怡新,黄凤玉,王海苹..基于网络药理学与实验验证研究仁青芒觉通过表观遗传调控轴PRMT1/H4R3me2a/PAI-1/MCP-1缓解肝纤维化的作用和机制[J].中国中药杂志,2025,50(18):5026-5035,10.基金项目
青海省中央引导地方科技发展项目(2023ZY025) （2023ZY025）
中国中医科学院中药研究所技术研发项目(20230301) （20230301）
云南省教育厅民族药物质基准研究重点实验室项目(2022YGZ02) （2022YGZ02）
云南省中青年学术和技术带头人后备人才项目(202205AC160039) （202205AC160039）
