浙江大学学报（理学版）2025，Vol.52Issue(6)：766-775,10.DOI:10.3785/j.issn.1008-9497.2025.06.010
碳酸酐酶菌的筛选固定化及其酶活性质研究
Screening,immobilization,and enzymatic properties research of carbonic anhydrase producing bacteria
摘要
Abstract
Carbonic anhydrase is an enzyme that efficiently catalyzes the hydration of CO2.To overcome its drawbacks of easy deactivation and poor recyclability,carbonic anhydrase-producing bacteria were immobilized and studied for enzyme activity properties.Bacteria capable of producing carbonic anhydrase were isolated from Karst soil and identified through 16S rDNA sequencing and phylogenetic analysis as belonging to the genus Stenotrophomonas sp.Using sodium alginate-chitosan composite materials as carriers,the enzyme activity recovery ratio before and after immobilization was used as an indicator of immobilization effectiveness.Experimental results showed an enzyme activity recovery rate of up to 55%.Response surface methodology was applied to explore the effects of immobilization time,sodium alginate concentration,chitosan concentration,and calcium chloride concentration on enzyme activity recovery rate,revealing the optimal immobilization conditions:2 hours of immobilization time,2%sodium alginate concentration,1%chitosan concentration,and 1 mol·L-1 calcium chloride concentration.It is shown that under these optimal conditions,the stability of the immobilized strain was significantly improved,and reusability was optimized.关键词
CO2捕集/碳酸酐酶/菌株鉴定/固定化细菌/响应面分析法Key words
CO2 capture/carbonic anhydrase/identification of bacteria/immobilized bacteria/response surface methodology分类
生物科学引用本文复制引用
李倩玮,刘浩,安雪娇,朱燕萍,龚灵俐..碳酸酐酶菌的筛选固定化及其酶活性质研究[J].浙江大学学报（理学版）,2025,52(6):766-775,10.基金项目
中央高校基本科研业务费专项资金资助项目(2462023BJRC006). （2462023BJRC006）
