浙江大学学报（农业与生命科学版）2025，Vol.51Issue(5)：772-783,12.DOI:10.3785/j.issn.1008-9209.2024.07.051
皖临白山羊ELF5基因克隆、表达分析及功能鉴定
Cloning,expression analysis,and functional identification of ELF5 gene in Wanlin white goat(Capra hircus)
摘要
Abstract
In order to clarify the gene sequence,structure and expression of E74 like ETS transcription factor 5(ELF5)in goat mammary gland and the effect of ELF5 gene on β-casein(CSN2)expression,this study took Wanlin white goat mammary epithelial cells as the experimental model and cloned the coding region sequence of ELF5 gene by polymerase chain reaction.The expression patterns of ELF5 gene in goat mammary gland at early,peak,middle,late and dry lactations were detected,and bioinformatics analysis was conducted using online software.After overexpression and inhibition of ELF5 gene,casein-related genes were detected.The results showed that the complete coding region of goat ELF5 gene is 768 bp long and encodes 255 amino acids.The molecular weight of the protein is 30 089.83 Da,and the theoretical isoelectric point is 5.61.There are 31 phosphorylation sites,without signal peptide and transmembrane helix structure.ELF5 is an unstable protein carrying negative charge.ELF5 interacts with prolactin receptor(PRLR),β-casein,estrogen related receptor beta(ESRRB),forkhead box A1(FOXA1),and so on.Goat ELF5 gene is closely associated to sheep and cattle.The expression levels of ELF5 and CSN2 genes were greater in early,peak and middle lactations of goats.In goat mammary epithelial cell models,overexpression of ELF5 increased the expression level of β-casein,whereas interference with ELF5 had the opposite effect,suggesting that ELF5 gene could promote β-casein synthesis in goat mammary epithelial cells.This study provides a theoretical basis for the study on the regulatory mechanism of β-casein synthesis by ELF5 gene during goat lactation.关键词
皖临白山羊/ELF5/基因克隆/生物信息学分析/β-酪蛋白Key words
Wanlin white goat/ELF5/gene clone/bioinformatics analysis/β-casein分类
农业科技引用本文复制引用
余雅杰,郭玉柱,马存霞,土彤彤,刘洪瑜,张运海,宋宁..皖临白山羊ELF5基因克隆、表达分析及功能鉴定[J].浙江大学学报（农业与生命科学版）,2025,51(5):772-783,12.基金项目
安徽省高校自然科学研究项目(KJ2021A0150) （KJ2021A0150）
安徽农业大学自然科学青年基金项目(K2139002) （K2139002）
安徽农业大学人才科研资助项目(rc392111). （rc392111）
