广东海洋大学学报2025，Vol.45Issue(5)：122-129,8.DOI:10.3969/j.issn.1673-9159.2025.05.016
产高活性壳聚糖酶菌株CJB-H6的筛选、鉴定及其酶学性质
Screening and Identification of Strain CJB-H6 Producing High-Activity Chitosanase and Enzymatic Properties of Its Chitosanase
摘要
Abstract
[Objective]This study aims to screen a high-yield chitosanase-producing strain and investigate the enzymatic properties of the chitosanase produced by this strain,as well as optimize the process conditions for the efficient preparation of chitosan oligosaccharides.[Methods]High-yield chitosanase strains from the sediments in the Yanwei Port area of Lianyungang were screened using the transparent zone method and enzyme activity assay;and chitosanase was purified by fractional precipitation and chromatography,and its enzymatic properties were characterized;single-factor experiments were conducted to optimize the conditions for the efficient production of chitosan oligosaccharides by chitosanase,using hydrolysis rate and oligosaccharide yield as indicators.[Results and Conclusion]Bacillus sp.CJB-H6 with chitosanase activity of(245.22±3.18)U/mL was obtained.The purified chitosanase from Bacillus sp.CJB-H6(Bpt CSN)had a molecular mass of approximately 34 kDa and a specific activity of(9 889.33±47.21)U/mg.Its optimal catalytic temperature and pH were 55℃and 6.0,respectively.Metal ions Ba2+and Mg2+significantly enhanced the activity of Bpt CSN(P<0.05).Under the conditions of 55℃,the optimal process conditions for Bpt CSN catalyzing 60 g/L colloidal chitosan are as follows:enzyme amount of 150 U/mL,acetic acid volume fraction of 2.0%,stirring speed of 100 r/min,and hydrolysis time of 2.5 hours,thereafter,the hydrolysis rate and oligosaccharide yield reached 94.69%±5.38%and 91.06%±4.91%,respectively.The hydrolysis products are mainly composed of chitobioses,chitotrioses,and chitotetraoses.关键词
壳聚糖酶/产酶菌株/芽孢杆菌属/酶学性质/壳寡糖Key words
chitosanase/enzyme producing strains/Bacillus sp./enzymatic properties/chitooligosaccharides分类
化学化工引用本文复制引用
陶甜甜,焦学,甘萍,俞国豪,崔仟,潘明轩,胡亚东,杨光..产高活性壳聚糖酶菌株CJB-H6的筛选、鉴定及其酶学性质[J].广东海洋大学学报,2025,45(5):122-129,8.基金项目
江苏省高等学校基础科学研究面上项目(24KJB180003) （24KJB180003）
江苏省青年科技人才托举工程项目(JSTJ-2024-168) （JSTJ-2024-168）
江苏省海洋资源开发技术创新中心海洋科技项目(LWKJ-07) （LWKJ-07）
江苏海洋生物资源创新中心海洋创新项目(JOUH23604) （JOUH23604）
