浙江农林大学学报2025，Vol.42Issue(5)：888-897,10.DOI:10.11833/j.issn.2095-0756.20250432
农杆菌介导的植物非组培遗传转化技术研究进展
Research progress on Agrobacterium-mediated genetic transformation technology of plant non-tissue culture
摘要
Abstract
Plant genetic transformation technology is a key means in modern plant gene function research and molecular breeding.However,the use of traditional methods for genetic transformation is highly dependent on plant tissue culture,and there are obvious genotype limitations and other problems.Agrobacterium-mediated non-tissue culture genetic transformation technology has emerged as a new form,which is gradually applied in a variety of plants due to its simple operation and no need to undergo dedifferentiation and regeneration.This paper systematically sorts out the basic principles of Agrobacterium-mediated genetic transformation and various methods of non-tissue culture transformation,including seed-mediated method,flower organ transformation method,living injection delivery method,cutting-impregnation-budding method,virus delivery method,electroporation method,etc.Other non-tissue culture genetic transformation methods independent of Agrobacterium were also summarized,including nanoparticle-assisted delivery,developmental regulator-assisted transformation,pollen tube-mediated transformation,and viral vector-mediated transformation.By comparing application examples,transformation efficiencies,advantages and disadvantages,and suitable plant species across different methods,it clearly demonstrates the potential of non-tissue-culture transformation systems in enhancing genetic transformation efficiency,broadening the range of applicable species,and lowering the technical barriers required for transformation.The purpose is to provide corresponding theoretical reference and technical support for the establishment of efficient,stable and universal plant non-tissue culture genetic transformation.[Ch,2 tab.71 ref.].关键词
非组培/根癌农杆菌/遗传转化/综述Key words
non-tissue culture/Agrobacterium/genetic transformation/review分类
农业科技引用本文复制引用
李娇娇,王晓光,白梦岚,侯小改,郭丽丽..农杆菌介导的植物非组培遗传转化技术研究进展[J].浙江农林大学学报,2025,42(5):888-897,10.基金项目
河南省中药产业技术体系(2024-23) （2024-23）
