浙江农林大学学报2025，Vol.42Issue(5)：934-943,10.DOI:10.11833/j.issn.2095-0756.20250422
百合原生质体分离与培养研究进展
A review on isolation and culture of lily protoplasts
摘要
Abstract
The highly heterozygous genetic background of Lilium spp.(lily)leads to complex trait segregation and difficulties in fixing superior genotypes.Coupled with the long growth cycle and widespread interspecific and intergeneric incompatibility barriers in distant hybridization,the efficiency of traditional hybridization breeding methods in the genetic improvement of lilies is severely limited.Research on the isolation and culture of lily protoplasts can provide technical support for somatic hybridization breeding,bypassing the barriers of sexual reproduction and creating interspecific and even intergeneric somatic hybrids,thus opening up new approaches for the utilization of distant genetic resources.This review summarizes the research progress on the isolation and culture of lily protoplasts over the past few decades,mainly focusing on the following aspects:(1)Factors affecting the isolation and purification of lily protoplasts,including tissue sources for protoplast isolation,pretreatment of isolation materials,enzymatic digestion conditions of protoplasts,osmotic pressure and pH of the digestion solution,and purification methods of protoplasts;(2)Factors influencing the cultivation of lily protoplasts,including composition of the protoplast culture medium,methods of protoplast culture,and density of protoplast culture;(3)Domestic and international cases of lily protoplast regeneration and analysis.Currently,the establishment of isolation and purification systems for lily protoplasts is relatively mature,but there are limited cases of complete plant regeneration from protoplast culture,and there are still significant bottlenecks in the establishment of regeneration systems.Future research should focus on systematically optimizing lily protoplast culture conditions,including the type of culture medium,carbon source types,and combinations and ratios of plant growth regulators.On the basis of establishing a stable and efficient regeneration system,the excellent traits of distant species can be introduced into cultivated varieties through protoplast fusion technology.At the same time,the mechanisms of regeneration and stable inheritance should be analyzed and applied to somatic hybridization,gene editing,and other breeding technologies.[Ch,1 tab.59 ref.].关键词
百合/原生质体分离/原生质体培养/原生质体再生Key words
Lilium spp./protoplast isolation/protoplast culture/protoplast regeneration分类
农业科技引用本文复制引用
陈兴旺,韩炘,陈敏敏,杨云尧,聂功平,张永春,明凤,杨柳燕..百合原生质体分离与培养研究进展[J].浙江农林大学学报,2025,42(5):934-943,10.基金项目
上海市农业科技创新项目(沪农科K2023-02-08-00-12-F04593) （沪农科K2023-02-08-00-12-F04593）
上海市专业技术服务平台能力提升项目(21DZ2292300) （21DZ2292300）
上海市科技兴农项目(沪农科推字[2022]第4-1号) （沪农科推字[2022]第4-1号）
