浙江农林大学学报2025，Vol.42Issue(5)：956-966,11.DOI:10.11833/j.issn.2095-0756.20250486
不同梅花品种花色表型与色素成分分析
Analysis of flower color phenotypes and pigment components in different Prunus mume cultivars
摘要
Abstract
[Objective]This study investigates the pigment composition of different cultivar groups of Prunus mume and its impact on flower color variation,thereby providing a foundation for targeted breeding strategies focused on floral coloration.[Method]Fifteen different flower color cultivars of P.mume were selected.The flower color phenotypes of the petals were determined using the Royal Horticultural Society Color Chart of the United Kingdom.The ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)technique was employed to qualitatively and quantitatively analyze the flavonoid compounds in the petals of P.mume.The correlations between the flower color phenotypes and pigment components were analyzed through cluster analysis,correlation analysis,principal component analysis,and multiple linear regression.[Result]As results,cluster analysis categorized the flower color phenotypes of the 15 P.mume cultivars into four groups:white,light pink,pink,and purplish-red.Qualitative and quantitative analysis of flavonoids revealed that purplish-red,pink,and light pink cultivars contained anthocyanins,primarily cyanidin and peonidin glycosides,with cyanidin-3-O-rutinoside being the most abundant.In contrast,white cultivars lacked anthocyanins but exhibited substantial accumulation of other flavonoids,such as isoquercitrin.Principal component analysis indicated that anthocyanins(PC1 contribution rate:40.64%)were the core pigments responsible for red hues in P.mume.Notably,red-green chromaticity(a*)showed a significant positive correlation with peonidin-3-O-rutinoside(P＜0.05),while higher total anthocyanin content correlated with lower lightness(L*),resulting in darker flower coloration.Other flavonoids(PC2 contribution rate:19.87%)played a secondary regulatory role.The multiple regression model confirmed the dominance of anthocyanins in color determination:total anthocyanins negatively correlated with L*(P＜0.01),peonidin-3-O-rutinoside was a key contributor to a*,and peonidin-3-O-glucoside showed a negative correlation with yellow-blue chromaticity(b*,P＜0.05).[Conclusion]The differentiation of P.mume flower colors is governed by a metabolic network characterized by"anthocyanin-driven pigmentation and flavonoid co-regulation".Cyanidin and peonidin glycosides are core components for formation of red hue,while other flavonoids likely play secondary roles in pigment stability or environmental adaptation.[Ch,8 fig.2 tab.34 ref.].关键词
梅花/品种/花色表型/花青苷/类黄酮Key words
Prunus mume/cultivars/flower color phenotypes/anthocyanin/flavonoid分类
农业科技引用本文复制引用
夏梦雪,杨钰,宁奥祥,肖政,兰延钢,赵宏波,王艺光..不同梅花品种花色表型与色素成分分析[J].浙江农林大学学报,2025,42(5):956-966,11.基金项目
国家重点研发计划项目(2023YFD2300900) （2023YFD2300900）
中央财政林业科技推广示范项目([2025]TS02) （[2025]TS02）
浙江省农业新品种选育重大科技专项(2021C02071) （2021C02071）
