浙江农林大学学报2025，Vol.42Issue(5)：984-993,10.DOI:10.11833/j.issn.2095-0756.20250429
野生玫瑰与重瓣白玫瑰杂交一代耐盐性评价及耐盐SNP筛选
Evaluation of salt tolerance and screening of salt-tolerant SNP markers in F1 generation derived from wild Rosa rugosa×R.rugosa'Alba Plena'
摘要
Abstract
[Objective]This study aims to provide a parental selection strategy for salt-tolerant breeding of Rosa rugosa,with the goal of enhancing breeding efficiency.[Method]F1 generation seedlings were first obtained by crossing wild R.rugosa with R.rugosa'Alba Plena'.The salt tolerance of these seedlings was then evaluated under salt stress conditions.Resequencing and high-resolution melting(HRM)genotyping were employed to screen and validate single nucleotide polymorphisms(SNP)associated with salt tolerance.[Result]Among the 83 F1 seedlings,more than 50%exhibited moderate or higher salt tolerance,with 12 individuals showing strong salt tolerance.Resequencing and SNP analysis of all hybrid progenies detected a total of 11 412 504 SNP loci,among which cytosine(C)＞thymine(T)and guanine(G)＞adenine(A)were the most frequent mutation types.The largest proportion of SNP(approximately 34.46%)were located in intergenic regions.Furthermore,5 well-genotyped SNP loci and their corresponding primers were screened.Among them,type Ⅰ of SNP63 could effectively eliminate salt-sensitive individuals,demonstrating its potential for marker-assisted selection in breeding for salt tolerance in R.rugosa.[Conclusion]The 12 salt-tolerant R.rugosa F1 generation exhibiting stronger salt tolerance than their parent lines were obtained,and a significantly salt tolerance-associated SNP marker(SNP63)was preliminarily identified,which will significantly enhance breeding efficiency.[Ch,5 fig.5 tab.25 ref.].关键词
玫瑰/杂交一代(F1)/耐盐性/单核苷酸多态性(SNP)Key words
R.rugosa/F1 generation/salt tolerance/single nucleotide polymorphisms(SNP)分类
农业科技引用本文复制引用
徐勇,毛一茹,马远潇,叶银秋,陈锈熔,王建文,冯立国..野生玫瑰与重瓣白玫瑰杂交一代耐盐性评价及耐盐SNP筛选[J].浙江农林大学学报,2025,42(5):984-993,10.基金项目
国家自然科学基金资助项目(32002076) （32002076）
