组织工程与重建外科杂志2025，Vol.21Issue(5)：458-466,9.DOI:10.3969/j.issn.1673-0364.2025.05.008
华通胶衍生静电纺丝制备人工血管支架在血管重建中的应用研究
Application of Wharton's jelly-derived electrospun artificial vascular grafts in vascular reconstruction research
摘要
Abstract
Objective To explore the feasibility of preparing electrospun artificial vascular grafts using Wharton's jelly(WJ)for promoting vascular reconstruction.Methods Fresh umbilical cords were voluntarily donated,and WJ was isolated and decellularized with parallel trypsinization.The decellularized WJ was then mixed with polycaprolactone(PCL)and electrospun to prepare WJ/PCL electrospun artificial vascular scaffolds.PCL electrospun artificial vascular scaffolds were prepared using the same method as a control.Liquid displacement method was used to determine the porosity,static contact angle test was used to detect hydrophilicity,BCA protein assay kit was used to evaluate protein adsorption,and weighing method was used to determine in vitro degradation performance.Human umbilical vein endothelial cells(HUVECs)were co-cultured for 7 days,and cell viability was assessed using live/dead cell staining and cell counting kit-8(CCK-8).Subcutaneous immune response assessment was conducted in rabbits(immunofluorescence staining for CD45 and CD163).Ten New Zealand white rabbits were used to prepare a 1 cm defect in the left carotid artery.PCL and WJ/PCL electrospun artificial blood vessels were used for repair(n=5 each).Doppler ultrasound and gross observation were performed 1 month postoperatively to assess vessel lumen diameter and patency.Biomechanical properties,and histology(Hematoxylin and Eosin staining and Masson's trichrome staining),as well as immunohistochemistry staining[smooth muscle cells(SMCs),α-smooth muscle actin(α-SMA),and endothelial cells(CD31)]were used for evaluating vascular structure.Results The experiment successfully prepared tubular PCL and WJ/PCL electrospun artificial vascular scaffolds.Compared to PCL electrospun artificial vascular scaffolds,the WJ/PCL scaffolds exhibited better hydrophilicity,protein adsorption,in vitro degradation rate,and cell compatibility(P<0.05),with lower immune response(P<0.05).Animal experiments showed that 1 month postoperatively,compared to the PCL group,the WJ/PCL group had higher carotid artery patency rate and larger lumen diameter(P<0.05).Gross observation showed a whiter repaired segment of vascular vessel tissue.Biomechanical evaluation showed higher ultimate tensile stress and fracture elongation.There was no severe granulation tissue hyperplasia,and more homogeneous and mature fibrous connective tissue was observed.The expression of SMCs,α-SMA,and CD31 was increased,with statistically significant differences(P<0.05).Conclusion The WJ/PCL electrospun artificial vascular scaffolds prepared based on WJ effectively promotes the repair of rabbit carotid artery defects.关键词
华通胶/人工血管/静电纺丝/组织工程/血管重建Key words
Wharton's jelly/Artificial vascular prosthesis/Electrospun/Tissue engineering/Vascular reconstruction分类
生物科学引用本文复制引用
孙定国,徐勇..华通胶衍生静电纺丝制备人工血管支架在血管重建中的应用研究[J].组织工程与重建外科杂志,2025,21(5):458-466,9.基金项目
上海市科技启明星计划(24QB2704800). （24QB2704800）
