生物资源2025，Vol.47Issue(5)：482-493,12.DOI:10.14188/j.ajsh.20250616001
一株高效产IAA枳壳内生真菌菌株的筛选鉴定及其促生作用
Screening and identification of an efficient endophytic fungal strain producing IAA in Citrus aurantium and its growth-promoting effect
摘要
Abstract
To explore the growth-promoting effects of functional endophytic fungal strains on plants,35 endophytic fungi strains previously isolated from Citrus aurantium L.were tested by Salkowski colorimetric method to screen for strains capable of producing indole-3-acetic acid(IAA).Morphological characteristics and rDNA ITS sequence analysis were combined to taxonomically identify the target strains.Inoculation and pot experiments were conducted to verify their growth-promoting effects.The results showed that one functional strain(ZYY007)with high-efficiency IAA production was successfully screened from the 35 endophytic fungi.Morphological and molecular identification confirmed that this strain belonged to Fusarium proliferatum.Orthogonal experimental design was used to optimize the fermentation condi-tions for strain ZYY007.The optimal parameters for IAA production were determined as follows:inoculum size of 7 fun-gal blocks(diameter 7 mm),fermentation temperature of 24 ℃,and shaking speed of 180 r/min.Under these condi-tions,the IAA yield in the fermentation broth reached 48.079 μg/mL;Growth-promoting assays showed that the fermen-tation broth of strain ZYY007 at medium and low concentrations significantly enhanced wheat seed germination rate and promoted the growth of radicles and plumules.Pot experiments further confirmed that treatment with this microbial agent significantly increased plant height,dry weight,and chlorophyll content of wheat seedlings.This study provides a theoret-ical basis and high-quality microbial resources for the development of novel microbial agents in agricultural production.关键词
枳壳/内生真菌/吲哚-3-乙酸/促生作用Key words
Citrus aurantium L./endophytic fungi/indole-3-acetic acid/growth-promoting effect分类
农业科技引用本文复制引用
黄忠祥,余雅思,孙惠,闵捷,欧阳建萍,陈晔,何刚..一株高效产IAA枳壳内生真菌菌株的筛选鉴定及其促生作用[J].生物资源,2025,47(5):482-493,12.基金项目
江西省药品监督管理局科研项目(2022JS06 （）
2023JS32) （）
江西省大学生创新创业训练计划项目(S202411843058) （S202411843058）
