生物资源2025，Vol.47Issue(5)：494-503,10.DOI:10.14188/j.ajsh.20250317002
基于三代转录组测序的湖北黄精SUVH基因家族鉴定与分析
Identification and analysis of SUVH gene family of Polygonatum zanlanseianense based on third-generation transcriptome sequencing
摘要
Abstract
Histone methylation,as one of the main mechanisms of epigenetic regulation,plays a key role in plant growth,development and environmental response.Based on the full-length transcriptome data of Hubei Huangjing(Po-lygonatum zanlanscianense)assembled by third-generation sequencing technology,the SUVH gene family in histone methyltransferase of Polygonatum zanlanscianense were identified and analyzed,including the sequence characteristics,protein structure,and expression pattern of SUVH family genes,and a protein evolution tree was constructed.The re-sults showed that a total of 39 SUVH genes were identified,with the number of amino acid residues ranging from 161 aa to 1 268 aa,the isoelectric point ranging from 4.77 PI to 9.10 PI,and the molecular weight ranging from 18 kDa to 142 kDa;among them,there were 20 genes encoding acidic proteins and 19 genes encoding basic proteins.Only the protein encoded by transcript/6593.p1 had a transmembrane domain in this gene family.This gene encoded a total of 837 amino acids,and amino acids from 813 to 835 formed a transmembrane helix.The SUVH gene family of Polygonatum zanlan-scianense had significant evolutionary conservation with Arabidopsis homologous genes,but the number of its genes was significantly greater than that of Arabidopsis.These results suggested that Polygonatum zanlanscianense may adapt to the complex secondary metabolism regulation needs through gene family expansion.Subcellular localization results showed that 76.47%of the protein family members were localized in the nucleus.The results of motif analysis showed that the conserved termination element at the 3'end may be involved in the regulation of substrate recognition specificity.This study analyzes the evolution and functional characteristics of the SUVH gene family in Polygonatum zanlanscianens,provides key gene resources for clarifying its epigenetic regulatory network and the synthesis mechanism of medicinal sec-ondary metabolism,and establishes a systematic method for the functional genomics research of medicinal plants.关键词
湖北黄精/组蛋白甲基化/基因家族分析/生物信息学/表观遗传学Key words
Polygonatum zanlanscianense Pamp./histone methylation/gene family analysis/bioinformatics/epi-genetics分类
生物科学引用本文复制引用
丁露,董伊洛,田科伟,张德春..基于三代转录组测序的湖北黄精SUVH基因家族鉴定与分析[J].生物资源,2025,47(5):494-503,10.基金项目
多组学解析玄参悬浮细胞培养合成环烯醚萜的调控机制研究(WDCM2023014) （WDCM2023014）
