生物资源2025，Vol.47Issue(5)：513-525,13.DOI:10.14188/j.ajsh.20250623001
聚多曲霉CMC-1的筛选鉴定及其水稻秸秆降解特性
Screening and identification of Aspergillus sydowii CMC-1 and its degradation characteristics of rice straw
摘要
Abstract
Crop straw is a cheap and abundant renewable resource,but its difficulty of degratation limits the efficien-cy of its high-value conversion and utilization.Screening highly efficient straw-degrading microbes is of great significance for the treatment of crop straw waste,the protection of the ecological environment,and the efficient utilization of biomass resources.A fungal strain CMC-1 with high-efficiency in degrading rice straw was screened from rotten straw soil using the sole carbon source method.This strain could grow well on four kinds of sole-carbon-source media,including rice straw,microcrystalline cellulose,sodium carboxymethyl cellulose,and xylan,respectively.Based on the phylogenetic analysis of its ITS sequence and the macroscopic and microscopic phenotypic characteristics,CMC-1 was identified as As-pergillus sydowii.Strain CMC-1 exhibited complete lignocellulolytic enzyme activities.In a fermentation medium with 2%wheat bran and 2%microcrystalline cellulose,the highest activity of β-glucosidase detected could reach 169.7 U/mL,and the highest activity of xylanase was 207.2 U/mL in shake flask fermentation at 30℃with a rotation speed of 180 rpm.The optimal pH for endo-β-glucanase,exoglucanase,and β-glucosidase was 5,while xylanase was 6.The optimal temperature for all four enzymes was 50℃.Cu2+could significantly promote the activities of both cellulase and hemicellulase.The degradation efficiency of CMC-1 on rice straw was 25.8%detected by the weight-loss method,and the degradation efficiencies of cellulose and hemicellulose were 24.2%and 26.9%,respectively.The Aspergillus sy-dowii CMC-1 screened in this study shows high degradation capacity for rice straw,which can provide a new excellent strain for the efficient conversion and utilization of agricultural straw biomass resources.关键词
水稻秸秆/唯一碳源/聚多曲霉/纤维素酶/半纤维素酶Key words
rice straw/sole carbon source/Aspergillus sydowii/cellulase/hemicellulase分类
生物科学引用本文复制引用
赵路兴,王亚蕊,李永康,崔钰华,尹守亮..聚多曲霉CMC-1的筛选鉴定及其水稻秸秆降解特性[J].生物资源,2025,47(5):513-525,13.基金项目
唐山市科技计划项目(24150212C) （24150212C）
华北理工大学高层次人才科研启动项目(BS201833) （BS201833）
