草业学报2025，Vol.34Issue(11)：98-113,16.DOI:10.11686/cyxb2025052
藜麦种子可培养内生真菌鉴定及分析
Identification and analysis of culturable endophytic fungi from quinoa seeds
摘要
Abstract
Quinoa(Chenopodium quinoa)is a newly introduced crop in China,and it is attracting much interest because of its high nutritional value.In this study,we aimed to clarify the population structure and functional characteristics of endophytic fungi in quinoa seeds.Culturable endophytic fungi were isolated from seeds of four varieties of quinoa.The fungi were cultured,identified on the basis of morphological and phylogenetic analyses,and their pathogenicity and antifungal effects were explored.In total,nine culturable endophytes were isolated from the quinoa seeds.They comprised nine species of six genera,namely,Alternaria alternata,Aspergillus flavus,Aspergillus sydowii,Fusarium boothii,Fusarium clavum,Neocamarosporium betae,Neocamarosporium chenopodii,Penicillium oxalicum,and Talaromyces stollii.A.alternata was present in seeds of all four varieties,and had the highest isolation frequency(64.42%).The fungi with the next highest isolation frequencies were A.flavus and P.oxalicum(11.66%and 9.20%,respectively).Among the nine culturable endophytic fungi,five(F.boothii,F.clavum,A.alternata,N.chenopodii,and N.betae)were able to infect quinoa leaves,and two(P.oxalicum and T.stollii)showed inhibitory effects against quinoa pathogens(Botrytis cinerea,Ascochyta caulina,Fusarium citri,A.alternata,Trichothecium roseum).The culturable endophytic fungi in quinoa seeds showed a rich population structure and have potential applications in the biological control of plant diseases.关键词
藜麦/可培养内生真菌/种群/系统发育Key words
quinoa/culturable endophytic fungi/population/phylogeny引用本文复制引用
秦楠,曹瑞鹏,高婧涵,彭玉飞,田淼,吕红,任璐,殷辉,赵晓军..藜麦种子可培养内生真菌鉴定及分析[J].草业学报,2025,34(11):98-113,16.基金项目
山西农业大学"科技创新提升工程"项目(CXGC2023009),山西省重点研发计划(2022ZDYF117),山西省现代农业产业技术体系建设专项(2025CYJSTX03-31)和"特""优"农业高质量发展科技支撑工程项目(TYGC25-01,TYGC25-02)资助. （CXGC2023009）
