草业学报2025，Vol.34Issue(11)：114-124,11.DOI:10.11686/cyxb2024515
基于SSR标记的象草F1代杂种分子鉴定及表型变异分析
Identification of hybrids and analysis of phenotypic variation in the F1 generation of Pennisetum purpureum based on SSR markers
摘要
Abstract
In plant breeding,it is important to generate hybrids with sufficient phenotypic variation for germplasm innovation and variety improvement.In this study,we used the traditional hybridization method to cross Pennisetum purpureum'GM1'(female parent)with the new line'F-B'(male parent),producing 536 hybrid offspring.We selected 150 representative F1 lines for variation analysis,and performed hybrid identification and phenotypic variation analysis based on phenotypic traits and simple sequence repeat(SSR)markers.The main findings were as follows:1)The F1 generation showed greater phenotypic variation than the parents.Leaf length exhibited significant hybrid vigor and trait differences,with variation coefficients of 5.90%-21.05%;2)Fourteen selected SSR primers amplified 219 clear bands,of which 118 were polymorphic,representing a polymorphism rate of 54.18%.The polymorphism information content value for SSR loci ranged from 29.59%to 44.63%(mean 35.40%);3)A cluster analysis based on phenotypic data grouped the hybrids into three categories,while SSR-based analysis divided them into four.The SSR-based analysis confirmed the maternal parent's genetic dominance;4)Specific primers accurately identified true hybrids with a 98%success rate.Most true hybrids had complementary parental bands,and some were the paternal-type.These results show that SSR analysis can reveal parental genetic information for the F1 generation.Analyses based on phenotypic traits and 15 SSR core primers were able to effectively identify authentic hybrids among 150 F1 hybrid progenies,highlighting materials that will be suitable for breeding new lines.关键词
象草/杂种F1代/杂种鉴定/SSR分子标记/表型分析/遗传多样性Key words
Pennisetum purpureum/F1 hybrid progenies/hybrid identification/SSR molecular marker/phenotypic analysis/genetic diversity引用本文复制引用
张献芳,聂刚,黄思源,余帅,左粟田,张新全..基于SSR标记的象草F1代杂种分子鉴定及表型变异分析[J].草业学报,2025,34(11):114-124,11.基金项目
国家现代农业产业技术体系(CARS-34)和四川省"十四五"饲草育种攻关项目(2021YFYZ0013)资助. （CARS-34）
