草业学报2025，Vol.34Issue(11)：125-135,11.DOI:10.11686/cyxb2024517
紫花苜蓿CKX基因家族鉴定及其对非生物胁迫的响应
Identification of the CKX gene family in alfalfa and its responses to abiotic stress
摘要
Abstract
Alfalfa(Medicago sativa)is the most widely planted legume forage in the world,with a high yield,strong stress resistance,and good palatability.Low temperature and drought are important abiotic stress factors affecting the yield,quality,and distribution of alfalfa.Cytokinin oxidase/dehydrogenase(CKX)irreversibly degrades cytokinin,thereby regulating plant growth and development,and it also contributes to the plant response to environmental stress.In this study,the CKX gene family of alfalfa was identified and analyzed by bioinformatics methods at the whole-genome level.In total,31 CKX genes were identified in the alfalfa genome,distributed on chromosomes 1,2,3,4,7,and 8.Members of the CKX gene family members encoded polypeptides of 273-545 amino acids,all of which were hydrophilic proteins except for MsCKX30.In a phylogenetic analysis,the CKX gene family grouped into three subfamilies with highly conserved domains,and alfalfa CKX genes showed high homology with those of Medicago truncatula.Analyses of gene transcript levels by quantitative real-time polymerase chain reaction showed that MsCKX2,MsCKX3,MsCKX7,MsCKX8,MsCKX15,MsCKX16,and MsCKX18 were induced under low-temperature stress,MsCKX2 and MsCKX15 were induced under drought stress,and MsCKX15 was induced by both low temperature stress and drought stress.These results provide a reference for further research on the functions of CKX genes in alfalfa.关键词
紫花苜蓿/CKX基因家族/生物信息学分析/表达分析Key words
alfalfa/CKX gene family/bioinformatics analysis/expression analysis引用本文复制引用
边林,张岩,霍晓伟,代蕊,郭娜,伊风艳,高翠萍,张志强..紫花苜蓿CKX基因家族鉴定及其对非生物胁迫的响应[J].草业学报,2025,34(11):125-135,11.基金项目
内蒙古自治区高等学校"青年科技英才支持项目"(NJYT23009),内蒙古一流学科专项项目(YLXKZX-NND-033),国家自然科学基金(32060388)和苜蓿分子育种体系构建及种质创制(BR22-11-12)资助. （NJYT23009）
