中国耳鼻咽喉头颈外科2025，Vol.32Issue(9)：579-584,6.DOI:10.16066/j.1672-7002.2025.09.008
慢性鼻窦炎上皮间质转化与TGF-β1/Smad通路表达及手术预后的相关性研究
Epithelial-mesenchymal transition and TGF-β1/Smad signaling in chronic rhinosinusitis and the correlation with surgical prognosis
摘要
Abstract
OBJECTIVE To compare the expression characteristics of transforming growth factor-β1(TGF-β1),Smad2,Smad3,and epithelial-mesenchymal transition(EMT)-related markers(E-cadherin,N-cadherin,vimentin)in patients with different types of chronic sinusitis(CRS),to analyze the correlations of E-cadherin,N-cadherin and vimentin with TGF-β1 and the prognosis of surgical treatment in patients with different types of CRS.METHODS The expressions of E-cadherin,N-cadherin,vimentin,TGF-β1,Smad2 and Smad3 in patients with different types of CRS and the control group were compared by Western blotting(WB)and real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).Analyze its correlation with the improvement degree of each clinical score after the operation.RESULTS Compared with the control group,the expression of E-cadherin decreased in the CRSsNP group,the non-ECRSwNP group and the ECRSwNP group,while the expressions of vimentin and N-cadherin increased.The protein expression of TGF-β1 in the CRSsNP group was higher than that in the non-ECRSwNP group and the control group(P<0.001),and the expressions of Smad2 and Smad3 in the CRSsNP group were higher than those in the ECRSwNP group,the non-ECRSwNP group and the control group(P<0.001).In the CRSsNP group,there was a positive correlation between TGF-β1 and vimentin(r=0.675,P=0.011),and a negative correlation with E-cadherin(r=-0.802,P=0.001).The expression of E-cadherin was negatively correlated with the improvement amplitude of SNOT-22 nasal symptom scores in patients with different types of CRS(P<0.05).CONCLUSION The EMT phenomenon occurs in different types of CRS.In CRS SNPS,EMT may be related to the TGF-β1/Smad signaling pathway.The expressions of EMT markers E-cadherin,N-cadherin and vimentin are correlated with the decrease in the severity of postoperative disease in patients with CRS,suggesting a potential association between the EMT process and the surgical prognosis of patients with CRS.关键词
鼻窦炎/鼻息肉/转化生长因子β1/上皮间质转化Key words
Sinusitis/Nasal Polyps/Transforming Growth Factor beta1/epithelial mesenchymal transition引用本文复制引用
石元贞,厚媛,刘龙燕,李勇,陈兴建,刘增平,段世宏..慢性鼻窦炎上皮间质转化与TGF-β1/Smad通路表达及手术预后的相关性研究[J].中国耳鼻咽喉头颈外科,2025,32(9):579-584,6.基金项目
甘肃省自然科学基金(24JRRA372) （24JRRA372）
兰州市科技计划项目(2023-2-81) （2023-2-81）
