东南园艺2025，Vol.13Issue(4)：394-400,7.DOI:10.20023/j.cnki.2095-5774.2025.04.009
红掌高效离体培养技术研究
Study on efficient in vitro culture technique of Anthurium andraeaum
摘要
Abstract
[Objective]The efficient in vitro propagation technology of Authurium andraeaum 'Pink Champion' was studied in order to provide a certain experimental basis for the factory seedling.[Method]The effects of the different explants on the callus induction and the effects of the different culture media on callus differentiation,proliferation and rooting were studied by using the aseptic seedlings of Authurium andraeaum 'Pink Champion' as the experimental materials.[Result]Cultured for 60 days in the same induction medium,the callus induction rate of stem segments(93.33%)was the highest.When the callus was cultured for 60 days and 90 days,the differentiation rate of adventitious buds in the medium of MS+0.25-0.50 mg/L 6-BA was significantly higher than that of MS+1.00 mg/L 6-BA.The callus cultured for 90 days without cutting were re-inoculated on the old formula medium for 100 days,the differentiation coefficient of adventitious buds and the dry matter proliferation coefficient in the medium of MS+0.25 mg/L 6-BA were the highest.The rooting rate of 1/2 MS basic medium was significantly higher than that of MS basic medium.And the rooting rate of rootless seedlings reached 100%when cultivated in the medium of 1/2 MS+0.20 mg/L NAA+1.00 g/L activated carbon(AC)for 12 days.[Conclusion]In the efficient in vitro propagation of Authurium andraeaum 'Pink Champion',stem segments were most suitable for inducing callus.The medium of MS+0.25 mg/L 6-BA was most suitable for callus differentiation and proliferation.The medium of 1/2 MS+0.20 mg/L NAA+1.00 g/L AC was most suitable for rooting.关键词
红掌/茎段/组织培养/离体繁殖Key words
Anthurium andraeaum/stem segments/tissue culture/in vitro propagation分类
园艺学与植物营养学引用本文复制引用
汤红玲,马晓娟,刘靓,陈品品,郑金水..红掌高效离体培养技术研究[J].东南园艺,2025,13(4):394-400,7.基金项目
福建省科技计划项目(2019N0048) （2019N0048）
