东南园艺2025，Vol.13Issue(4)：410-416,7.DOI:10.20023/j.cnki.2095-5774.2025.04.011
七指毛桃离体快繁体系建立与优化
Establishment and optimization of in vitro rapid propagation system of Ficus hirta Vahl
摘要
Abstract
[Objective]Ficus hirta Vahl is an important medicinal and edible plant in the Lingnan region of China.In order to break through the bottleneck of large-scale and industrialized production technology of Ficus hirta Vahl,an efficient regeneration and cultivation technology system for Ficus hirta Vahl is established.[Method]This study established a tissue culture and rapid propagation technology system for Ficus hirta Vahl by optimizing the sterilization system of explants,hormone ratio,and transplanting substrate.[Result](1)Pretreatment with 75%ethanol for 30 s combined with 0.1%HgCl2 sterilization for 8 min reduced the contamination rate to 22.1%;(2)Adding 2.0 mg/L 6-BA and 0.5 mg/L NAA to WPM medium was most beneficial for inducing adventitious buds,with an induction rate of 38.7%;Adding 2.0 mg/L 6-BA and 0.5 mg/L NAA into the proliferation medium could promote the proliferation of adventitious buds,with a proliferation coefficient of 3.13;The rooting medium(1/2 WPM+0.5 mg/L IBA+0.2 mg/L NAA)achieved a rooting rate of 91.2%;(3)The matrix with a peat soil:vermiculite:perlite ratio of 3:1:1 achieved a transplant survival rate of 86.5%and a new leaf germination rate of 78.2%by balancing porosity and slow-release nutrient characteristics.[Conclusion]The establishment of an efficient regeneration and cultivation technology system for Ficua hirta Vahl provides technical support for the large-scale production of Ficus hirta Vahl seedlings and the cultivation of authentic medicinal materials,and provides theoretical reference for the optimization of in vitro regeneration system of Ficus medicinal plants.关键词
七指毛桃/组培/消毒/培养基配方/移栽/药食同源Key words
Ficus hirta Vahl/tissue culture/sterilization/culture medium formula/transplant/homology of medicine and food分类
农业科技引用本文复制引用
陈汉鑫,韦幂,林艺辉,马馨怡,王锦玲..七指毛桃离体快繁体系建立与优化[J].东南园艺,2025,13(4):410-416,7.基金项目
福建省科技计划项目(2023N0054) （2023N0054）
