福建林业科技2025，Vol.52Issue(3)：31-38,8.DOI:10.13428/j.cnki.fjlk.2025.03.004
闽楠炭疽病病原菌的分离与鉴定
Isolation and Identification of the Pathogen Causing Anthracnose on Phoebe bournei
摘要
Abstract
In this study,leaf samples of Phoebe bournei showing anthracnose symptoms were collected from the nursery of Youxi County Forestry Bureau in Sanming City,Fujian Province.The pathogen was isolated via tissue isolation and confirmed by Koch's postulates,then identified through morphological characteristic observation and multi-locus sequence(ITS,CHS-1,GAPDH,ACT,and TUB2)phylogenetic analysis.Results showed that 18 isolates were obtained from the diseased P.bournei seedlings,which could be classified into two categories(representative strains:YX-1,YX-2)based on morphological characteristics.Verification through Koch's postulates demonstrated that both strains were pathogenic to P.bournei seedlings.Morphological identification showed that the initial colonies of YX-1 were white.After 4-5 days,the center turned gray,with a loose felt-like texture,dense aerial hyphae,neat edges,and a pale yellow reverse side.The initial colonies of YX-2 were white,with a layer of white cotton-like aerial hyphae cover-ing the surface.After 4-5 days,the center became light gray while the edges remained white or grayish-white.Orange-red conidial heaps formed in the center,and the reverse side was pale yellow,accompanied by the secretion of black dot-like pigments.Conidia of both strains were long elliptical,with obtuse ends,colorless,transparent,unicellular,and smooth-surfaced.The size of YX-1 conidia ranged from 13.1～17.3 μm × 3.0～6.5 μm,while that of YX-2 conidia ranged from 14.0～23.5 μm × 3.0～4.5μm.Phylogenetic analysis indicated that YX-1 clustered with Colletotrichumfructicola(node support rate 100％),and YX-2 with C.siamense(node support rate 98％).Integrating morphological and phylogenetic results,YX-1 was identified as C.fructicola,and YX-2 as C.siamense.This research provides a theoretical basis and practical reference for the accurate diagnosis and scientific con-trol of P.bournei anthracnose.关键词
闽楠/炭疽病/病原鉴定/系统发育分析/致病性Key words
Phoebe bournei/anthracnose/pathogen identification/phylogenetic analysis/pathogenicity分类
林学引用本文复制引用
詹芳芳,王子豪,陈艳彬,邱细妹,曾丽琼,张毅锋,蔡守平..闽楠炭疽病病原菌的分离与鉴定[J].福建林业科技,2025,52(3):31-38,8.基金项目
福建省科技计划引导性项目(2024N01010004) （2024N01010004）
国家林业和草原局南方山地用材林培育重点实验室资助 （）
