福建农林大学学报（自然科学版）2025，Vol.54Issue(6)：740-746,7.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202411022
国兰光合碳同化途径对干旱胁迫的响应
Response of photosynthetic carbon assimilation pathways in Chinese Cymbidium to drought stress
摘要
Abstract
[Objective]The photosynthetic carbon assimilation pathways in Chinese Cymbidium species and their physiological adap-tations to drought stress were investigated for the establishment of scientific foundation for water management strategies in cultivation and conservation.[Method]Five primary Chinese Cymbidium species,C.sinense,C.faberi,C.goeringii,C.ensifolium and C.kanran,were firstly analyzed regarding stable carbon isotope fractionation values(δ13C)of the photosynthetic leaves.Then diurnal net CO2 absorption dynamics and dawn-dusk titratable acid accumulation were quantified under regular irrigated condition as the control(9％-12％moisture content)and drought stress(0％moisture content at day 7)for interspecific comparative analyses.[Result](1)The δ13C values of all species ranged from-30.92‰ to-27.81‰,in line with the characteristics of C3 photosynthetic plants.(2)Nocturnal CO2 assimilation was increased under drought stress,with C.sinense,C.faberi,C.goeringii,C.ensifolium and C.kanran up by 13.17,3.29,2.42,1.68 and 0.93 μmol·m-2,respectively.(3)C.goeringii,C.faberi and C.kanran exhibited significantly pos-itive dawn-dusk titratable acid differences(ΔH+)both under drought-stressed condition and the control,specifically up from 3.20 to 13.50 mmol·kg-1 in C.goeringii,from 6.27 to 10.70 mmol·kg-1 in C.faberi,and from 7.27 to 2.30 mmol·kg-1 in C.kanran,con-trastly C.ensifolium displayed significantly negative shift from-1.34 to-4.00 mmol·kg-1,only C.sinense demonstrated marginal in-crease from 1.00 to 1.40 mmol·kg-1.[Conclusion]The 5 Chinese Cymbidium species belong to the facultative crassulacean acid metabolism(CAM)taxa within C3 plants.Drought stress upregulated the expression of CAM pathway,with the highest induction ob-served in C.goeringii and C.faberi,followed by C.sinense,while C.kanran and C.ensifolium exhibited the lowest expression levels.关键词
兰属/干旱胁迫/碳同化途径/景天酸代谢/光合特性Key words
Cymbidium/drought stress/carbon assimilation pathway/crassulacean acid metabolism/photosynthetic characteristics分类
生物学引用本文复制引用
简惠玲,吴宇涵,许静,朱梦佳,兰思仁,李明河..国兰光合碳同化途径对干旱胁迫的响应[J].福建农林大学学报（自然科学版）,2025,54(6):740-746,7.基金项目
福建省自然科学基金杰出青年项目(2024J010023). （2024J010023）
