福建农林大学学报（自然科学版）2025，Vol.54Issue(6)：758-768,11.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202411033
闽楠ARF基因家族的鉴定及其在花器官中的表达分析
Identification of ARF gene family and analysis on its expression patterns in floral organs of Phoebe bournei
摘要
Abstract
[Objective]The structure,function and expression patterns of auxin response factor(ARF)in the floral organs of Phoebe bournei were investigated at different developmental stages,to provide insights into the molecular mechanisms underlying the regula-tion of floral organ development.[Method]Bioinformatics methods were utilized to identify and analyze the ARF gene family at ge-nome-wide level,and then transcriptome data were integrated to examine the expression patterns of these genes in floral organs at different developmental stages.[Result]A total of 24 PbARFs were identified and classified into 3 distinct subfamilies by phylogenet-ic analysis.Analyses on the intron-exon structure and conserved motifs not only showed their high conservativeness in evolution but also supported the classification of PbARF subfamilies.Overal,the PbARFs exhibited different expression patterns in various tissues,and the high expressions of PbARF1/2/3/14/15/16/18 in the tepals,pistils and stamens indicated their positive regulatory roles in the maturation of P.bournei floral organs,specifically with the high expression of PbARF18 in the stamens.[Conclusion]The PbARF gene family played significant roles in the development and maturation of P.bournei flowers,especially PbARF1/2/3/14/15/16/18,which were likely to exert positive regulatory function in the flower development and pollination process.关键词
生长素响应因子/闽楠/基因家族/花器官/分子育种Key words
auxin response factor/Phoebe bournei/gene family/floral organs/molecular breeding分类
农业科学引用本文复制引用
毕远洋,付厚华,翁慧莹,尤丽梅,江子涵,林文俊,陈世品..闽楠ARF基因家族的鉴定及其在花器官中的表达分析[J].福建农林大学学报（自然科学版）,2025,54(6):758-768,11.基金项目
福建省林业科技推广项目(2022TG16) （2022TG16）
福建农林大学科技创新专项基金项目(KFB23047). （KFB23047）
