福建农林大学学报（自然科学版）2025，Vol.54Issue(6)：800-809,10.DOI:10.13323/j.cnki.j.fafu(nat.sci.).202410028
华南沿海3种常见牡蛎的GDF-8基因克隆与表达分析
Molecular cloning and expression analysis of GDF-8 gene of 3 common oyster species in south China coastal areas
摘要
Abstract
[Objective]This study investigated the expression patterns and function of the GDF-8 gene in Crassostrea hongkongensis,Crassostrea rivularis,and Crassostrea sikamea,to provide a theoretical foundation for unveiling oyster muscle development and main-tenance.[Method]The core coding sequences(CDS)of GDF-8 gene from 3 kinds of oyster were obtained by polymerase chain reac-tion(PCR),and followed by bioinformatics analysis.The expression patterns of GDF-8 in different tissues were detected by real-time fluorescence quantitative PCR(qRT-PCR).[Result]The CDS of GDF-8 gene in the 3 oyster species were 1 317 bp in length,and encoded 438 amino acids.The encoded GDF-8 protein all included TGF-β superfamily domain,and shared homology of 95.48%-96.83%with the GDF-8 of C.gigas.The expressions of ChGDF-8,CarGDF-8 and CsiGDF-8 were the highest in the labrum,which were consecutively declined in the adductor muscle,digestive gland,mantle and gills of C.hongkongensis.The expression of CarG-DF-8 was higher in the mantle of C.rivularis and lowest in the gill.The CsiGDF-8 was highly expressed in the gill of C.sikamea,and least expressed in the adductor muscle.[Conclusion]GDF-8 was involved in the development and maintenance of muscle in adult oysters.关键词
GDF-8基因/香港牡蛎/近江牡蛎/熊本牡蛎/基因克隆Key words
GDF-8 gene/Crassostrea hongkongensis/Crassostrea rivularis/Crassostrea sikamea/gene cloning分类
水产学引用本文复制引用
农珍珍,周易,朱鹏,严雪瑜,刘玥,钟方杰,蒋美君,蒙扬辉,陈颖,陈肇聪,黄龙兴,丁彬洋..华南沿海3种常见牡蛎的GDF-8基因克隆与表达分析[J].福建农林大学学报（自然科学版）,2025,54(6):800-809,10.基金项目
北部湾大学引进高层次人才科研启动项目(2019KYQD39) （2019KYQD39）
广西水产遗传育种与健康养殖重点实验室开放项目(GXKEYLA2020-01) （GXKEYLA2020-01）
钦州市科学研究与技术开发计划项目(20223637、20221214). （20223637、20221214）
