福建农业科技2025，Vol.56Issue(8)：62-68,7.DOI:10.13651/j.cnki.fjnykj.2025.08.010
正丁醇增强卡那霉素杀灭耻垢分枝杆菌的效果分析
Effect Analysis of N-butanol on Enhancing Kanamycin's Killing Efficacy Against Mycobacterium smegmatis
摘要
Abstract
Mycobacterium smegmatis,as a classic model strain for tuberculosis research,exhibits the characteristics of intrinsic drug resistance and clinical pathogenicity,which can cause the epidermal and soft tissue infections.In order to overcome its intrinsic drug resistance barrier,Mycobacterium smegmatis was treated with n-butanol and kanamycin at different concentrations and different treatment times.The survival rate of bacteria was determined,and the integrity of cell membrane was detected by PI staining.The results showed the combination of 0.5 mol·L-1 n-butanol and kanamycin achieved the optimal synergistic bactericidal efficacy.The combined treatment of 10 min significantly improved the bactericidal efficiency.The synergistic effect exhibited both concentration-independent and rapidly responsive characteristics.N-butanol could significantly enhance the antibacterial activity of kanamycin and streptomycin against Mycobacterium smegmatis,and showed a significant synergistic effect on these two antibiotics.The PI staining showed that the fluorescence intensity of bacteria in the combined treatment group was significantly higher than that in the single-agent treatment group,indicating that the cell membrane permeability was significantly increased.The study could provide a reference for developing the novel adjuvant drugs for anti-tuberculosis drugs.关键词
正丁醇/卡那霉素/耻垢分枝杆菌/存活率Key words
N-butanol/Kanamycin/Mycobacterium smegmatis/Survival rate分类
医药卫生引用本文复制引用
张裕丹,翁丽红,高媛媛..正丁醇增强卡那霉素杀灭耻垢分枝杆菌的效果分析[J].福建农业科技,2025,56(8):62-68,7.基金项目
福建省自然科学基金面上项目(2024J01295). （2024J01295）
