福建农业学报2025，Vol.40Issue(8)：767-776,10.DOI:10.19303/j.issn.1008-0384.2025.08.003
辽棉系列品种黄萎病抗性综合评价
Verticillium Wilt Resistance of Liaomian Cottons
摘要
Abstract
[Objective]The resistance of Liaomian cotton to verticillium wilt was studied to aid disease control.[Methods]In 2022-2023,29 varieties of Liaomian cotton in Liaoning Province were evaluated for their resistance to verticillium wilt using multiple means including field disease nursery evaluation,in vitro Verticillium dahliae Vd991 inoculation on seedlings,and staining assay on burst of reactive oxygen species(ROS)in seedling stems and Morks as control.Standard data transformation-Euclidean distance with sum of squared deviations cluster analysis was applied to classfication the selected varieties.[Results]The Liaomian series of cotton cultivars displayed significant resistance to the wilt at the nurseries in Liaoyang,Liaoning and Shijiazhuang,Hebei.Among them,13 varieties were rated resistant(R)or highly resistant(HR).Although the indices of the in vitro inoculation test on the seedlings were relatively high,12 of them were shown to be tolerant of the disease,as similarly indicated in the field test.In addition,the NBT and DAB staining on the seedling stems after ROS burst suggested that 11 varieties to be wilt resistant.Overall,LM19,LM23,LM25,LM27,LM33,LM40,and LM42 displayed stable and considerate resistance to verticillium wilt.The cluster analysis using the Euclidean distances of 3.25 and 4.0 classified the 29 cotton varieties into 5 categories.By comparing the distribution and revealed that the disease resistance and breeding time of varieties in each cluster group,revealed that the disease resistance increased with delay of breeding time.[Conclusion]The varieties of Liaomian cotton carried verticillium wilt resistance in the genetic pool.关键词
辽棉品种/黄萎病/抗病性/室内苗期鉴定/活性氧(ROS)爆发染色Key words
Liaomian cotton/verticillium wilt/disease resistance/in vitro seedling determination/staining test for burst of reactive oxygen species分类
农业科学引用本文复制引用
朱鹤,李越,单莹,王子胜,徐敏..辽棉系列品种黄萎病抗性综合评价[J].福建农业学报,2025,40(8):767-776,10.基金项目
国家农业生物育种重大专项(2023ZD04041) （2023ZD04041）
