福建农业学报2025，Vol.40Issue(8)：800-809,10.DOI:10.19303/j.issn.1008-0384.2025.08.006
马铃薯早疫病病原菌茄链格孢菌Alternaria solani线粒体基因组特征分析
Mitogenome Characterization of Potato Early Blight Pathogen Alternaria solani
摘要
Abstract
[Objective]The mitochondrial genome of a strain of Alternaria solani,the primary pathogen of early blight disease in potatoes,was assembled and characterized.[Methods]DNA was extracted and sequenced from A.solani TA-0410 to study the mitogenome structure to compare with other similar strains for a clustering analysis.[Results]The mitochondrial genome was 58 494 bp with a G+C content of 28.80%with 35.40%of A nucleotides,35.80%of T,13.90%of G,and 14.90%of C.It contained 24 protein-coding and 25 tRNA genes.The SSR analysis identified 224 short repeat sequences ranging from 2 bp to 8 bp in length.The 6 most frequently occurring short repeats were AT with 146 occurrences,followed by AAT with 26,AG with 25,AC with 14,AAG with 4,and ACT with 4 occurrences.In 29 387-29 419 bp of the genome,4 copies of 8 bp repeat sequence of AATTATAT were found.A phylogenetic analysis based on 28 complete mitochondrial genomes indicated that A.solani was most closely related to the congeneric species,A.tenuissima and A.alternata,and relatively with 7 fungi from genera such as Stemphylium lycopersici,Curvularia clavate,Exserohilum rostratum,Exserohilum turcicum,Bipolaris cookie,Bipolaris oryzae and Bipolaris sorokiniana.The TA-0410 mitochondrial genome exhibited a significant codon usage bias that was primarily driven by natural selection and mutational pressure,as 47.62%of the codons had a RSCU greater than 1 with 90.63%ending in A or U,while 50.79%had the value less than 1 with 93.75%ending in C or G.[Conclusion]The mitogenome of A.solani displayed an extensive collinearity with those of several other Alternaria species.Phylogenetically,they were evolutionarily close despite the significant bias on genetic codon usage due primarily to natural selection and domestication.关键词
茄链格孢/马铃薯早疫病/线粒体基因组/聚类分析/密码子偏好性Key words
Alternaria solani/potato early blight/mitogenome/cluster analysis/codon bias分类
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刘文雯,杨巧梅,吴彦瑢,王红美,余雨功,冯雨蝶,李健美,唐唯..马铃薯早疫病病原菌茄链格孢菌Alternaria solani线粒体基因组特征分析[J].福建农业学报,2025,40(8):800-809,10.基金项目
云南省科技厅基础研究专项(202301AS070010、2202201AT070026) （202301AS070010、2202201AT070026）
