福建农业学报2025，Vol.40Issue(8)：810-821,12.DOI:10.19303/j.issn.1008-0384.2025.08.007
xatB基因簇在水稻细菌性条斑病菌致病力调控中的作用
Regulatory Function of xatB Gene Cluster in Virulence of Xanthomonas oryzae pv.oryzicola
摘要
Abstract
[Objective]Role of xatB gene cluster played in regulating the infection of Xanthomonas oryzae pv.oryzacola(Xoc)on rice plants was investigated.[Method]Single,double,and triple mutants of XoconxatB,Xoc_1597(mfd),and Xoc_1599(rlmJ)in the cluster as well as the corresponding complemented strains were constructed.Pathogenicity of the mutated and complemented wild-type strains were detected by a punching method,and growth,extracellular enzyme activities,and motility determined by plate culture.[Result]All xatB,mfd,and rlmJ mutations resulted in a significant decline in the virulence of Xoc.However,the rlmJ mutation inhibited,while mfd promoted,the growth,motility,and extracellular polysaccharide(EPS)synthesis;but the mfd complementation retarded those functions of the pathogen.The double and triple mutants differed from the single mutants in phenotypes as well as antagonistic or synergistic effects on Xoc.[Conclusion]XatB,Mfd,and RlmJ significantly affected the Xoc virulence on rice plants.The complex relationship requires further investigation.关键词
水稻细菌性条斑病菌/乙酰转移酶/致病力/胞外酶/运动性Key words
Xanthomonas oryzaepv.oryzacola/acetyltransferase/virulence/extracellular enzymes/motility分类
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王美华,吴可建,陶均..xatB基因簇在水稻细菌性条斑病菌致病力调控中的作用[J].福建农业学报,2025,40(8):810-821,12.基金项目
国家自然科学基金项目(32260647) （32260647）
