福建农业学报2025，Vol.40Issue(8)：830-836,7.DOI:10.19303/j.issn.1008-0384.2025.08.009
南方根结线虫生防菌JXJA01鉴定及其杀线活性
Identification and Nematicidal Activity of Biocontrol Strain JXJA01 against Meloidogyne incognita
摘要
Abstract
[Objective]Identification and nematicidal activity against Meloidogyne incognita of an Actinomyces strain were investigated for root-knot disease control.[Method]An Actinomycete strain,JXJA01,previously isolated from the rhizosphere soil of Dioscorea alata Lirm.sp in Ganzhou,China was positively identified by morphological,physiological,and biochemical examinations as well as a molecular evolution analysis.The supernatant of JXJA01 fermentation broth was co-cultured with a suspension of the second stage parasite juveniles(J2)or fresh nematode eggs to determine the nematicidal activity of the isolated strain.[Result]JXJA01 was identified as Streptomyces europaeiscabiei.The 2-fold dilution of the supernatant of the bacterial fermentation broth displayed the strongest biological activity against J2s among all preparations with an adjusted mortality rate in 24 h of 95.53%.It also had a robust suppressive effect on egg-hatching with a relative inhibition rate of 44.00%after 7 d of treatment.On potted tomatoes,it showed 40.88%efficacy in controlling the root-knot nematodes in 60d and significantly increased the fresh weight of roots by 71.42%over control.Moreover,the fermentation supernatant was not sensitive to temperature or light and maintained the nematicidal activity in storage at 4℃for 21 d.[Conclusion]Identified as S.europaeiscabiei,JXJA01 exhibited a promising potential to be used as a biological agent for controlling root-knot nematodes on tomatoes.关键词
生防资源/根结线虫/放线菌/发酵上清液/杀线活性Key words
biocontrol agent/root-knot nematode/actinomycete/fermentation supernatant/nematicidal activity分类
植物保护学引用本文复制引用
范琳娟,张帆,吴彩云,徐雪亮,姚健,刘子荣,姚英娟..南方根结线虫生防菌JXJA01鉴定及其杀线活性[J].福建农业学报,2025,40(8):830-836,7.基金项目
江西省农业科学院基础研究与人才培养专项(JXSNKYJCRC202206) （JXSNKYJCRC202206）
