福建农业学报2025，Vol.40Issue(8)：837-847,11.DOI:10.19303/j.issn.1008-0384.2025.08.010
西藏墨脱地区番茄青枯菌的分离鉴定及生防菌剂效果评价
Isolation,Characterization,and Effective Biocontrol Agents of Ralstonia solanacearum Causing Tomato Bacterial Wilt in Medog,Tibet
摘要
Abstract
[Objective]The pathogen causing tomato bacterial wilt in Medog,Tibet was isolated,identified,genetic diversity clarified,and effective biocontrol agents determined.[Method]Potential pathogens that caused the bacterial wilt on tomato plants were isolated and purified from the specimens collected in Medog by tissue separation.Specific species were identified based on morphological characteristics and molecular analysis prior to toxicologic classification using the phylotype-specific multiplex PCR(Pmx-PCR),physiological race,and biovar determination.Partial egl sequences were amplified by PCR for phylogenetic analysis to assign sequevars.Inhibitory effects of 5 biocontrol agents against the isolated pathogen were assessed using the agar plate diffusion method(inhibition zone assay).[Result]Eight Ralstonia solanacearum strains were isolated from the sampled 6 tomato plants infected with bacterial wilt in Medog.All isolates belonged to Phylotype I,Biovar III,and Sequevar 48.In vitro inhibition tests on the isolate demonstrated significant efficacies of all 5 agents.Of them,Changbairun Microbial Agent showed the largest average inhibition zone diameter that exceeded 19 mm.[Conclusion]R.solanacearum strains from Medog were isolated,characterized,and population genetic structure defined.An effective biocontrol agent was identified for eco-friendly control of the disease on tomato plants.关键词
墨脱地区/青枯劳尔氏菌/番茄青枯病/遗传多样性/生防菌剂Key words
Medog region/Ralstonia solanacearum/tomato bacterial wilt/genetic diversity/biocontrol agents分类
植物保护学引用本文复制引用
王荣波,蔡松龄,张前荣,李本金,余知雨,何玉仙,姜帆,刘裴清..西藏墨脱地区番茄青枯菌的分离鉴定及生防菌剂效果评价[J].福建农业学报,2025,40(8):837-847,11.基金项目
福建省农业科学院科技创新团队建设项目(CXTD2021002-1) （CXTD2021002-1）
厦门市重大科技项目(3502Z20221018) （3502Z20221018）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021011、XTCXGC2021017) （XTCXGC2021011、XTCXGC2021017）
