分析测试学报2025，Vol.44Issue(11)：2223-2227,5.DOI:10.12452/j.fxcsxb.25012254
基于RPA-侧向流试纸条技术的单核细胞增生李斯特氏菌快检技术
Rapid Detection Technology for Listeria Monocytogenes Based on RPA-Lateral Flow Strip Technology
摘要
Abstract
In order to enable food production enterprises to conduct more efficient and convenient daily monitoring of foodborne pathogens in the production environment,this study developed a rapid detection technology for Listeria monocytogenes based on recombinase polymerase amplification(RPA)-lateral flow strip technology,and analyzed the specificity,sensitivity,and detection limit of the method.The sensitivity of the lateral flow strip detection was 6.5×102 CFU/mL after 15 min of RPA amplification.The detection limit was 1 CFU/mL after 8 hours of enrichment of artificially contaminat-ed samples.The rapid detection method for Listeria monocytogenes established in this study does not require the assistance of large experimental equipment,and can be completed within 20 minutes af-ter simple training for testing personnel,providing good technical support for enterprises to monitor Listeria monocytogenes in the production environment from the source,and also promoting the im-provement of food safety quality control levels in the entire industry.关键词
食源性致病菌/重组聚合酶扩增(RPA)-侧向流试纸条/单核细胞增生李斯特氏菌/食品安全质量控制水平Key words
foodborne pathogens/recombinase polymerase amplification(RPA)-lateral flow strip/Listeria monocytogenes/food safety quality control level分类
化学化工引用本文复制引用
曹悦,李龙,索一平,李庆尧,王青龙,张爽,邢超,宋丽萍..基于RPA-侧向流试纸条技术的单核细胞增生李斯特氏菌快检技术[J].分析测试学报,2025,44(11):2223-2227,5.基金项目
国家重点研发计划项目资助(SQ2022YFF1100056) （SQ2022YFF1100056）
