分析测试学报2025，Vol.44Issue(11)：2265-2276,12.DOI:10.12452/j.fxcsxb.241122552
煅牡蛎标准汤剂"量-质"传递规律研究
Research on the Law of"Quantity-Quality"Transfer of the Standard Decoction of Calcined Ostreae Concha
摘要
Abstract
By comprehensively applying modern analytical methods,taking the extract yield,the content of CaCO3,the transfer rate of CaCO3,as well as simultaneous thermal analysis(TG-DSC),Fourier transform infrared spectroscopy(FTIR),and high performance liquid chromatography(HPLC)fingerprints as key quality attributes,this study reveals the"quantity-quality"transfer rules of Ostreae concha,calcined slices of Ostreae concha,and the standard decoction of calcined Ostreae concha,providing a reference for their quality control.Fifteen batches of Ostreae concha me-dicinal materials were selected as raw materials to prepare the calcined slices of Ostreae concha and the standard decoction of calcined Ostreae concha.The extract yield of the standard decoction of cal-cined Ostreae concha was calculated.The complexometric titration method was used to determine the CaCO3 content of the three,and the transfer rate of CaCO3 from the calcined slices of Ostreae concha to the standard decoction was calculated.TG-DSC was used to study the thermal decomposition char-acteristics and thermal effect characteristics of the three,and TG-DSC fingerprints were construct-ed.FTIR was used to establish FTIR fingerprints to analyze the dynamic changes of chemical groups in the three.HPLC was used to determine the amino acid components of the three,and HPLC finger-prints were constructed,and similarity evaluation was carried out.At the same time,combined with principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA),the similarities and differences among the three in terms of FTIR characteristics and amino acid compo-nents were explored.The results showed that the CaCO3 contents of 15 batches of Ostreae concha,calcined slices of Ostreae concha,and the standard decoction of calcined Ostreae concha were 94.68％-97.79％,96.53％-98.41％,and 81.86％-90.21％,respectively.The extract yield of the standard decoction of calcined Ostreae concha was 2.78％-4.94％,and the transfer rate of CaCO3 from the calcined slices of Ostreae concha to the standard decoction was 2.54％-4.42％.The results of TG-DSC showed that the organic components and CaCO3 in Ostreae concha,the calcined slices of Ostreae concha and the standard decoction of calcined Ostreae concha decomposed within the tempera-ture ranges of 30-580 ℃ and 580-800 ℃ respectively.Compared with Ostreae concha and the cal-cined slices of Ostreae concha,the endothermic peak of CaCO3 in the standard decoction of calcined Ostreae concha appeared earlier,and its thermal stability decreased significantly.The FTIR results showed that the main component of the three was calcite-type CaCO3.The main groups in Ostreae con-cha were O—H,C=O,and CO32-groups,and the C=O group was not observed after calcination.The HPLC results showed that the average similarity of the established fingerprints was all above 0.9,and 14 amino acid components could be effectively transferred.In addition,the multivariate statisti-cal analysis method could effectively distinguish the three,and 7 FTIR differential bands and 3 ami-no acid differential components were screened out.The method established in this study can be used for the quality control of the whole process of the standard decoction of calcined Ostreae concha.关键词
煅牡蛎/标准汤剂/"量-质"传递规律/同步热分析/傅里叶红外光谱/高效液相色谱Key words
calcined Ostreae concha/standard decoction/quantity-quality transfer/simultane-ous thermal analysis/Fourier infrared spectroscopy/high performance liquid chromatography分类
化学化工引用本文复制引用
贾德政,毕晓黎,江洁怡,陈伟韬,张靖年,胥爱丽,谢灿辉,曾志浩,刘焰嫦,肖观林..煅牡蛎标准汤剂"量-质"传递规律研究[J].分析测试学报,2025,44(11):2265-2276,12.基金项目
广东省基础与应用基础研究基金项目(2022A1515220171) （2022A1515220171）
