分析测试学报2025，Vol.44Issue(11)：2285-2294,10.DOI:10.12452/j.fxcsxb.25021283
大蒜硫代亚磺酸酯抗氧化活性研究及含量测定
Antioxidant Activity and Content Determination of Thiosulfinates in Garlic
摘要
Abstract
This study investigated the in vitro antioxidant activity and analytical methods of thiosul-finate compounds in garlic,elucidating the relationship between antioxidant components and their structural characteristics.The antioxidant capacity of freeze-dried garlic powder and alliin extract was evaluated through 1,l-diphenyl-2-picryl-hydrazyl radical(DPPH)and 2,2'-azinobis(3-ethylben-zothiazoline-6-sulfonic acid)diammonium salt(ABTS)radical scavenging assays.The results demon-strated that thiosulfinates generated via enzymatic reactions exhibited significantly higher scavenging rates against DPPH and ABTS radicals compared to deactivated enzyme and non-enzymatic systems(p＜0.01).UPLC-MS/MS and HPLC analysis identified four thiosulfinate products:dimethyl thio-sulfinate,allicin,methyl allyl thiosulfinate,and allyl methyl thiosulfinate.Combined with thin-lay-er chromatography-bioautography,allicin,methyl allyl thiosulfinate,and allyl methyl thiosulfinate were identified as the primary antioxidant components.The 5,5'-dithiobis(2-nitrobenzoic acid)(DTNB)method was employed to determine the potential total thiosulfinate content,which showed a positive correlation with the antioxidant activity of the test samples and was directly related to the con-tent of the precursor alliin.This study established a comprehensive research framework for investigat-ing the"component-activity"relationship of thiosulfinates,providing theoretical foundations and innovative directions for the development of natural antioxidants and strategies for preventing and treating oxidative stress-related diseases.关键词
大蒜/硫代亚磺酸酯/大蒜辣素/抗氧化/薄层生物自显影Key words
garlic/thiosulfinates/allicin/antioxidant/thin-layer bioautography分类
化学引用本文复制引用
李世颖,刘睿婷,史荣梅,杜娟,马璇,肖赵辉,张露,李建光,李新霞..大蒜硫代亚磺酸酯抗氧化活性研究及含量测定[J].分析测试学报,2025,44(11):2285-2294,10.基金项目
新疆维吾尔自治区重大科技专项(2022A03007-2) （2022A03007-2）
新疆维吾尔自治区"天山英才"青年科技拔尖人才项目(2022TSYCCX0019) （2022TSYCCX0019）
新疆维吾尔自治区重点研发任务专项项目(2024B03038-3) （2024B03038-3）
