分析测试学报2025，Vol.44Issue(11)：2362-2368,7.DOI:10.12452/j.fxcsxb.25012149
基于UPLC-Q-Orbitrap-HRMS的地榆炒炭前后化学成分差异分析
Differences Analysis of Chemical Constituents in Sanguisorba Officinalis L. Before and After Carbonized Based on UPLC-Q-Orbitrap-HRMS
摘要
Abstract
This study applied ultra-performance liquid chromatography tandem quadrupole electro-static field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap-HRMS)to analyze the changes of chemical constituents of Sanguisorba officinalis L.before and after carbonized,and to screen the different hemostatic components.Waters BEH C18(100 mm×2.1 mm,1.7 μm)column was used to process with 0.1％formic acid water solution and acetonitrile as the mobile phase for gra-dient elution.The heated electrospray ion source(HESI)was adopted to collect the data in both posi-tive and negative ionization modes.Based on the accurate mass,fragment ions,literature and net-work database,a total of 41 chemical components were identified by matching precise molecular ion masses,MS/MS fragmentation patterns,literature data,and database references.These included 17 phenolic acids,12 flavonoids,9 triterpenoids,and 3 other compounds.Principal component analysis(PC A)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were conduct-ed using SIMCA 14.1 software to screen differential components between Sanguisorba officinalis L.before and after carbonization.After carbonization,the contents of hemostasis-enhancing components(gallic acid,ziyuglycoside Ⅱ,tormentic acid and ursolic acid)increased significantly,while glu-cogallin,catechin,brevifolin carboxylic acid,ellagic acid and pomolic acid decreased.The results showed that triterpenoids and phenolic compounds in Sanguisorba officinalis L.after carbonized may constitute the key material basis for its enhanced astringent hemostatic effect.This investigation pro-vides scientific insights into the intrinsic mechanisms underlying the strengthened hemostatic efficacy of Sanguisorba officinalis L.after carbonization.关键词
地榆/UPLC-Q-Orbitrap-HRMS/化学成分/中药炮制/高分辨质谱/差异分析Key words
Sanguisorba officinalis L./UPLC-Q-Orbitrap-HRMS/chemical constituents/Chi-nese medicine processing/high resolution mass spectrometry/differences analysis分类
化学化工引用本文复制引用
刘畅,周熙,刘倩宝,吴惠勤,罗辉泰..基于UPLC-Q-Orbitrap-HRMS的地榆炒炭前后化学成分差异分析[J].分析测试学报,2025,44(11):2362-2368,7.基金项目
广东省科学技术厅科技基础条件建设领域专项项目(2024B1212070001) （2024B1212070001）
广东省科学院发展专项资金项目(2022GDASZH-2022010110) （2022GDASZH-2022010110）
江门市科技特派员项目(2024760000280010017) （2024760000280010017）
