分析化学2025，Vol.53Issue(10)：1642-1653,12.DOI:10.19756/j.issn.0253-3820.251195
基于双电化学探针的纳米通道生物传感方法用于癌胚抗原检测
Nanochannels Electrochemical Biosensing Method Based on Dual Electrochemical Probes and Its Application in Detection of Carcinoembryonic Antigen
摘要
Abstract
A self-calibrating nanochannels electrochemical biosensing method for carcinoembryonic antigen(CEA)was established using two electrochemical probes,potassium hexacyanoferrate(K3[Fe(CN)6])and methylene blue(MB).Firstly,the electrochemical behaviors of K3[Fe(CN)6]and MB solutions were investigated,and it was found that these two probes could easily interact to form complexes.Based on this,K3[Fe(CN)6]and MB were used as electrochemical signal probes,respectively,CEA antibody-functionalized anodic aluminum oxide(AAO)nanochannels as recognition units,and DNA self-assembled hybridization chain reaction(HCR)as a signal amplification strategy to improve the sensitivity and accuracy of the detection.A self-calibrating nanochannels electrochemical biosensing method based on the two electrochemical probes was thus established.The optimal experimental conditions were 1.5 h of target incubation time,1 h of CEA aptamer incubation time,0.5 μmol/L of hybridization chain concentration and 2 h of hybridization chain reaction time.The linear range of this method for CEA detection was 0.01 ng/mL to 100 ng/mL,and the detection limits(S/N=3)of K3[Fe(CN)6]and MB as probes were 8.2 and 2.0 pg/mL,respectively.The recoveries for CEA determination in rat serum were closer to 100%after calibration.In addition,the established electrochemical biosensing method had good specificity,repeatability and selectivity.This study provided a new idea for improving the accuracy of nanochannel electrochemical biosensing analysis.关键词
铁氰化钾/亚甲基蓝/癌胚抗原/纳米通道/双探针/自校正Key words
Potassium hexacyanoferrate/Methylene blue/Carcinoembryonic antigen/Nanochannel/Dual probes/Self-calibrating引用本文复制引用
焦姣,张宏芳..基于双电化学探针的纳米通道生物传感方法用于癌胚抗原检测[J].分析化学,2025,53(10):1642-1653,12.基金项目
国家自然科学基金项目(No.21775120)和山西省高等学校科技创新项目(No.2025L107)资助. Supported by the National Natural Science Foundation of China(No.21775120)and the Scientific and Technological Innovation Program of Higher Education Institutions in Shanxi(No.2025L107). （No.21775120）
