分析化学2025，Vol.53Issue(10)：1694-1704,11.DOI:10.19756/j.issn.0253-3820.251018
基于电子微生物生长分析仪快速筛查食品中的活性沙门氏菌
An Electronic Microbial Growth Analyzer-based Method for Rapidly Screening Viable Salmonella in Food
摘要
Abstract
Foodborne illnesses caused by Salmonella pose significant threats to worldwide public health safety.In this study,a rapid method for screening viable Salmonella in oyster sauce and milk was developed by utilizing an electronic microbial growth analyzer(EMGA).Target food samples were diluted 10-fold with RVS broth and loaded into test tubes.Test tubes were positioned in the EMGA to determine the bacterial growth curves and the time required to reach the maximum growth rate(Tmgr).Using Salmonella typhimurium(S.typhimurium)asan model species,there was linear relationship between the logarithmic value of viable bacterial concentration(lgC)and Tmgr over the range of 5×101-5×106 CFU/mL,with a detection limit of 10 CFU/mL.For oyster sauce,the regression equation was Tmgr(min)=-80.775lg[C/(CFU/mL)]+754.96(R2=0.9907),and the recovery rates of S.typhimurium ranged from 95.2%to 119.8%,with relative standard deviations(RSD)ranging from 3.5%to 16.3%.For milk,the regression equation was Tmgr(min)=-71.922 lg[C/(CFU/mL)]+618.65(R2=0.9985),with recovery rates ranging from 98.4%to 110.6%and RSD ranging from 6.4%to 12.8%.The EMGA method required only one portable instrument,and involving only three manual steps,i.e.,dilution,transfer,and insertion.When S.typhimurium contamination reached 106 CFU/mL,the total time consumption,from the unwrapping of samples to the readout of bacterial concentration,was no more than 7 h.When applied to detection of actual oyster sauce and milk samples,the new method demonstrated strong consistency with plate counting results in positive detection rates.This method was superior to the plate counting method,which was generally considered as a gold standard,in terms of accuracy,precision,simplicity and efficiency,representing a promising alternative for the on-site screening and quantification of viable Salmonella in oyster sauce and milk products.关键词
食源性病原菌/活性沙门氏菌/筛查/定量快检/电子微生物生长分析仪Key words
Foodborne pathogens/Viable Salmonella/Screening/Rapid quantitative detection/Electronic microbial growth analyzer引用本文复制引用
梁若寒,李晨钟,刘珊,普小丹,卢峰,朱雪婷,张圆圆,王笑旸,杨倩倩,李昊,张旭志..基于电子微生物生长分析仪快速筛查食品中的活性沙门氏菌[J].分析化学,2025,53(10):1694-1704,11.基金项目
国家重点研发计划项目(No.2024YFD2400402)资助. Supported by the National Key Research and Development Program of China(No.2024YFD2400402). （No.2024YFD2400402）
