广东农业科学2025，Vol.52Issue(7)：1-8,8.DOI:10.16768/j.issn.1004-874X.2025.07.001
外施褪黑素对本生烟抗东亚西番莲病毒的效果及作用分析
Exogenous Melatonin for Nicotiana benthamiana Against East Asian Passiflora Virus(EAPV):Antiviral Efficacy and Function Analysis
摘要
Abstract
[Objective]The objective of this study is to elucidate the effects and to analyze the function of melatonin in enhancing resistance against East Asian Passiflora Virus(EAPV)in Nicotiana benthamiana.[Method]N.benthamiana plant were treated with various concentrations of melatonin(0,50,100,200,400 µmol/L),applied both before and after EAPV inoculation,healthy plants treated with melatonin-free solution and not inoculated with virus were used as control(CK).An optimal concentration was screened for by monitoring external symptoms,molecular virus detection,and changes in salicylic acid(SA)and jasmonic acid(JA)levels.Subsequently,the protective(anti-infection)and inhibitory(anti-replication)effects of melatonin were quantitatively assessed by measuring the host OD405 values.[Result]EAPV infection significantly reduced plant height.Untreated infected plants(T0)showed a height of 6.4(±0.20)cm,52.2%of CK.Foliar application of melatonin(200 µmol/L,T3)most effectively alleviated stunting.When inoculated at day 1,day 4,and day 7 post-treatment,infected plant heights were 1.66,1.27,and 1.06 times higher than T0,respectively.Despite failing to protect plants from infection(protection rate<0),200 µmol/L melatonin exhibited a substantial inhibitory effect on EAPV,suppressing viral accumulation by 26.9%on average.JA levels increased significantly from 10.38 ng/g(day 0)to 123.37 ng/g(day 7),while SA levels remained unchanged.[Conclusion]After inoculating EAPV,the plant significantly dwarfed.The application of exogenous melatonin could significantly alleviate the dwarfism of the native tobacco,with the optimal concentration being 200 µmol/L.After spraying melatonin,the JA level in the native tobacco significantly increased,while the SA level did not change significantly,suggesting that exogenous melatonin mitigates EAPV-induced stunting by activating JA-mediated induced systemic resistance(ISR),rather than directly preventing viral entry.关键词
褪黑素/本生烟/东亚西番莲病毒(EAPV)/诱导系统抗性/保护率/抑制率Key words
melatonin/Nicotiana benthamiana/East Asian Passiflora Virus(EAPV)/induced systemic resistance(ISR)/protection rate/inhibition rate分类
农业科技引用本文复制引用
王健华,尹慧祥,羊彬彬,冯小艳,余乃通..外施褪黑素对本生烟抗东亚西番莲病毒的效果及作用分析[J].广东农业科学,2025,52(7):1-8,8.基金项目
海南省自然科学基金(322RC765) （322RC765）
