广东农业科学2025，Vol.52Issue(7)：84-92,9.DOI:10.16768/j.issn.1004-874X.2025.07.008
湛江名龙黄鸡禽白血病抗性选育的研究
Research on the Selection Breeding of Resistance to Avian Leukemia in Zhanjiang Minglong Yellow Feather Broilers
摘要
Abstract
[Objective]To explore the resistant breeding situation of the receptor resistant alleles tva and tvb of A/K and B/D/E subgroups of avian leukosis virus within the 7th generation of the grandparental A,B and C lines of Zhanjiang Minglong Yellow feather broilers.[Method]A total of 900 blood samples from the A,B,and C strains of the 7th generation of the ancestral line of Zhanjiang Minglong Yellow feather broilers were subjected to PCR amplification of the regions containing the resistant loci of the tva and tvb receptor genes.The sequencing results were genotyped,and the genotype frequencies and allele frequencies of each resistant loci of the tva and tvb receptor genes were statistically analyzed.[Result]Genotyping of the resistant loci of the tva and tvb receptor genes in the 7th generation of the ancestral line of Zhanjiang Minglong Yellow feather broilers revealed that the tvar3,tvar4,tvar5 and tvbr3 resistant loci were present in all A,B and C lines.The highest frequency of the tvar3 resistant allele in hens of the A strain reached 0.417,and the highest frequency of the homozygous resistant genotype tvar5/r5 in roosters of the A strain reached 0.283;the highest frequency of the tvar5 resistant allele in hens of the B strain reached 0.367,and the highest frequency of the homozygous resistant genotype tvar4/r4 in roosters of the B strain reached 0.267;the highest frequency of the tvar3 resistant allele in hens of the C strain reached 0.423,and the highest frequency of the homozygous resistant genotype tvar3/r3 in roosters of the C strain reached 0.283.Among the A,B,and C strains,the frequency of the tvbr3 resistant allele in hens of the A strain was the lowest at 0.908,while that in hens of the C strain was the highest at 0.958.Among them,the frequency of the homozygous resistant genotype tvbr3/r3 in hens of the C strain was the highest at 0.917.[Conclusion]By integrating the research findings on the genetic diversity of tva and tvb receptor genes,the 7th grandparent stocks(Lines A,B,and C)of Zhanjiang Minglong Yellow feather broilers have demonstrated significantly enhanced resistance to avian leukosis virus subgroups ALV-A/K and ALV-B/D/E.Furthermore,a comprehensive breeding system for avian leukosis resistance in this breed has been successfully established for the first time.关键词
湛江名龙黄鸡/tva抗性位点/tvb抗性位点/遗传多态性/禽白血病/抗性选育Key words
Zhanjiang Minglong Yellow feather broilers/tva resistant loci/tvb resistant loci/genetic polymorphism/avian leukemia/resistance selection分类
农业科技引用本文复制引用
徐慧娟,胡思奇,陈伟国,谢青梅,邹子韬,谢欣怡,毕玉,苏琬婷,徐永健,李毅,李咏琪,李顺..湛江名龙黄鸡禽白血病抗性选育的研究[J].广东农业科学,2025,52(7):84-92,9.基金项目
农业农村部农业生物育种重大项目(2023ZD0405301) （2023ZD0405301）
广东省科技计划项目(2023B0202010029) （2023B0202010029）
华南农业大学大学生创新创业训练计划项目(202410564009,X202510564094) （202410564009,X202510564094）
