高等学校化学学报2025，Vol.46Issue(11)：30-38,9.DOI:10.7503/cjcu20250166
新型荧光碳点的一步法制备及细胞成像性能
One-step Preparation of Novel Fluorescent Carbon Dots for Cell Imaging
摘要
Abstract
In this study,novel fluorescent carbon dots(CDs)were rapidly synthesized via a one-step hydrothermal method using 1,8-naphthalic anhydride(NA)and 1,2-phenylenediamine(OPD)as precursors.By optimizing reaction conditions,yellow fluorescent carbon dots(Y3-CDs)with superior luminescence performance were prepared.The particle sizes and morphology of the Y3-CDs were characterized by high-resolution transmission electron microscopy(HRTEM),revealing an average diameter of approximately 2.6 nm and an interlayer spacing of 0.21 nm.This spacing corresponds to the d-spacing of graphene(100)planes and is consistent with the diffraction peak observed at 42.88o in X-ray powder diffraction(XRD)analysis.X-ray photoelectron spectroscopy(XPS)confirmed the presence of sp2/sp3 hybridized carbons(C=C/C—C,284.8 eV),carbon-nitrogen bonds(C—N,286.5 eV),and carbonyl carbons(C=O,288.4 eV).The optical properties of the Y3-CDs were further investigated using fluorescence spectrometry(FL),ultraviolet-visible absorption spectroscopy(UV-Vis),and lifetime measurements.The fluorescence spectra exhibited non-excitation-dependent emission behavior,indicating a single emission center.Fourier-transform infrared spectroscopy(FTIR)and nuclear magnetic resonance(NMR)provided detailed structural insights.Due to their high purity,the characteristic hydrogen(H)and carbon(C)atoms could be clearly resolved and analyzed.1H NMR results demonstrated that all H atoms were located on the surface of the carbon core,exhibiting ortho-substitution patterns and coupling effects.13C NMR and DEPT 135 spectra confirmed the presence of ten distinct tertiary carbon atoms.The cell-tracking and imaging performance of Y3-CDs were systematically evaluated.Results demonstrated that Y3-CDs possess excellent cell imaging capabilities and anti-photobleaching properties.Even after 180 minutes of continuous irradiation,Y3-CDs retained strong fluorescence,enabling long-term tracking of HeLa cells.Co-localization experiments further highlighted their selective lipid droplet imaging ability,with a Pearson's correlation coefficient of 0.846.关键词
碳点/核磁共振分析/抗光漂白/细胞示踪/脂滴成像Key words
Carbon dots/NMR analysis/Anti-photobleaching/Cell tracking/Lipid droplet imaging分类
化学化工引用本文复制引用
范红婷,徐佳彤,齐春轩,马恒昌..新型荧光碳点的一步法制备及细胞成像性能[J].高等学校化学学报,2025,46(11):30-38,9.基金项目
国家自然科学基金(批准号:22165026)和2025年甘肃省高校研究生"创新之星"项目(批准号:2025CXZX-275)资助. Supported by the National Natural Science Foundation of China(No.22165026)and the Innovative Star Project for Graduate Students in Gansu Province Universities 2025,China(No.2025CXZX-275). （批准号:22165026）
