干旱地区农业研究2025，Vol.43Issue(5)：47-58,78,13.DOI:10.7606/j.issn.1000-7601.2025.05.05
外源6-BA对盐碱胁迫下大豆种子和幼苗生理特性和相关基因表达的影响
Effects of exogenous 6-BA on physiological characteristics and related gene expression of soybean seeds and seedlings under saline-alkali stress
摘要
Abstract
This study investigated the mechanism of exogenous 6-benzyladenine(6-BA)in alleviating salt-alkali stress on soybean seed germination and seedling growth using two soybean cultivars,'Henong 95'and'Ni-uyanjing'.Through seed germination and seedling growth experiments,the effects of salt-alkali stress and 6-BA treatment on germination parameters,growth indicators,antioxidant systems,endogenous hormone regulation,and related gene expression were compared.The results showed that salt-alkali stress induced ROS accumulation in soy-bean seeds and seedlings,causing oxidative damage and significant reductions in radicle length(78.34％～81.00％),dry mass(52.31％～63.40％),radicle volume(67.37％～80.62％),radicle surface area(85.15％～85.20％),and root diameter(15.49％～45.52％)compared to controls.Application of 60 pumol·L-16-BA en-hanced salt-stress tolerance by regulating the antioxidant defense system and ROS accumulation,and improved ger-mination rate(33.33％),germination potential(7.32％～51.85％),germination index(20.76％～32.27％),and vigor index(2.80％～49.09％)in both cultivars.Exogenous 6-BA modified endogenous hormone levels(tZ,GA3,IAA,and ABA),increasing their contents by 7.85％～16.37％in'Henong 95'while decreasing them by 5.56％～12.77％in'Niuyanjing'.This treatment alleviated radicle growth inhibition by regulating cytokinin metabolism and signaling-related genes,significantly improving radicle dry matter mass(88.71％～145.55％),root volume(117.90％～465.00％),root surface area(78.64％～98.87％),and root diameter(124.82％～235.48％).Compared to salt-alkali treatment alone,6-BA application increased leaf color value(77.90％～89.62％)and stomatal conductance(118.85％～125.67％),effectively enhancing photosynthetic capacity and antioxidant activity in seedlings.These improvements promoted underground nodule formation and root growth,resulting in increased root length(1.55％～17.00％)and dry matter mass(70.43％～75.36％).关键词
大豆/6-BA/盐碱胁迫/种子萌发/幼苗/抗逆生理Key words
soybean/6-BA/saline-alkali stress/seed germination/seedling/anti-stress physiology分类
农业科学引用本文复制引用
李蔚佳,于淼,魏嘉谊,马世乐,周阳,吴许,关维明,杜吉到,杜艳丽..外源6-BA对盐碱胁迫下大豆种子和幼苗生理特性和相关基因表达的影响[J].干旱地区农业研究,2025,43(5):47-58,78,13.基金项目
省基金:东北北部高油大豆促油增产关键技术研究(2023ZD0403106) （2023ZD0403106）
黑龙江省自然科学基金联合引导项目(LH2024C077) （LH2024C077）
黑龙江八一农垦大学引进人才科研启动计划基金(XYB202006) （XYB202006）
省基金:耐盐碱大豆种质的应用及抗盐碱栽培技术示范(LJGX-CG2022-111) （LJGX-CG2022-111）
黑龙江八一农垦大学研究生创新科研项目(NXYYJSCX2023-Y06) （NXYYJSCX2023-Y06）
