干旱地区农业研究2025，Vol.43Issue(5)：59-68,10.DOI:10.7606/j.issn.1000-7601.2025.05.06
BrCTL1基因增强白菜型冬油菜抗寒性
BrCTL1 gene enhances the cold tolerance of Winter Brassica rapa L
摘要
Abstract
This study observed the ice crystal morphology of chitinase protein in the leaves of highly cold-re-sistant winter rapeseed through a microscope and analyzed the expression patterns of its encoding gene family under cold stress.The chitinase gene(BrCTL1)was cloned,the subcellular localization was carried out,and an overex-pression vector was constructed.The physiological changes and gene expression patterns of overexpressing lines un-der low-temperature stress were determined.The results showed that under cold-stress conditions,the protein solu-tion extracted from the leaves of highly cold-resistant winter rapeseed had significant recrystallization inhibition ac-tivity,effectively inhibits ice-crystal growth,and contains highly active antifreeze proteins.The protein was identi-fied as Chitinase(CTL1)by SDS-PAGE electrophoresis.The BrCTL1(Brapa08T003472.1)gene of rapeseed was cloned by the homologous cloning method.Its ORF was 963 bp in full length and encoded 321 amino acids.The BrCTL1 protein was located in the chloroplast.Under low-temperature stress,there were no obvious morphological changes in the BrCTL1 gene overexpression plants,while obvious freezing injury symptoms occurred in the mutant and wild-type plants.The activities of SOD,POD,and CAT in the overexpression Arabidopsis lines increased by 20％,17％,and 48％,respectively,compared with the wild-type,while those in the CTL1 mutant decreased by 65％,32％,and 67％,respectively.Under low-temperature stress,the relative conductivity and MDA content in the BrCTL1 overexpression lines decreased by 19％and 45％,respectively,compared with the wild-type,while those in the CTL1 mutant increased by 9.4％and 5.7％,respectively.In conclusion,the BrCTL1 gene can signifi-cantly enhance the cold resistance of transgenic Arabidopsis thaliana plants.关键词
BrCTL1基因/白菜型冬油菜/抗冻蛋白/几丁质酶/低温胁迫/抗寒性Key words
BrCTL1 gene/Winter Brassica rarp L./antifreeze protein/chitinase/low-temperature stress/cold resistance分类
农业科学引用本文复制引用
朱淑俊,撒二梅,李宝晶,魏鸿燕,罗倩,张欣怡,秦将源,梁欢,杨峰鹏,刘自刚,王小霞,魏家萍,崔俊美,武泽峰,王晋雄,方彦,董小云,郑国强..BrCTL1基因增强白菜型冬油菜抗寒性[J].干旱地区农业研究,2025,43(5):59-68,10.基金项目
国家自然科学基金(32360520) （32360520）
甘肃省自然科学基金重点项目(23JRRA1408) （23JRRA1408）
甘肃省科技重大专项(22ZD6NA009) （22ZD6NA009）
甘肃省重点人才项目(2023RCXM33) （2023RCXM33）
甘肃省科技计划项目(23CXJA0001) （23CXJA0001）
林芝市科技计划项目(LZZX-04) （LZZX-04）
