干旱地区农业研究2025，Vol.43Issue(5)：148-158,11.DOI:10.7606/j.issn.1000-7601.2025.05.15
垄沟覆膜和施磷对紫花苜蓿地土壤-植物-微生物系统生态化学计量的影响
Effects of ridge and furrow mulching and phosphorus application on the ecological stoichiometry of soil-plant-microorganism system in alfalfa field
摘要
Abstract
From 2011 to 2016,the alfalfa artificial grassland was used as the research object in Zhonglian-chuan village,Yuzhong County,Gansu Province.The randomized block of split-plot design was used.Two mulching methods(no mulching with flat planting and mulching with ridge and furrow)and four phosphorus appli-cation gradients(P0:no P applied,P1:apply 9.73 kg·hm-2 phosphorus,P2:apply 19.3 kg·hm-2 phosphorus,and P3:apply 28.9 kg·hm-2 phosphorus)were set up.During the period,soil and aboveground plant samples were collected every year to analyze the C,N,and P content of the soil-plant-microorganism system and their eco-logical stoichiometric characteristics.The results showed that,(1)In addition to high P treatment(P3),plastic film mulch with ridge and furrow averagely increased soil C,N,C∶N,C∶P,N∶P,soil microbial biomass C contents,C∶N,and C∶P by 39.3％,27.1％,13.5％,55.3％,49.8％,57.6％,39.6％,and 43.3％,respec-tively,compared to no mulching with flat planting.Soil available P under mulching with ridge and furrow averagely decreased by 13.9％,compared to no mulching with flat planting.(2)Soil C,C∶N,plant N,soil microbial bio-mass C,C∶N,and C∶P increased with increasing P levels in no mulching with flat planting but increased initial-ly and then decreased with the increasing P levels under mulching with ridge and furrow.(3)Plant C∶N and soil microbial biomass nitrogen(MBN)in P1,P2 and P3 treatments decreased by 2.94％,7.02％,11.5％and 8.62％,11.5％,19.1％,respectively,compared to P0 in no plastic film mulch with flat planting.Plant C∶N and MBN in P1 treatment decreased by 10.0％and 24.3％respectively compared to P0,while in P3 treatment increased by 8.20％,5.93％and 27.4％,9.92％,respectively,compared to P1 and P2 under mulching with ridge and furrow.(4)P fertilization respectively increased soil,plant,and soil microbial biomass P by 67.2％to 164.0％,14.0％to 34.3％,and 19.2％to 87.0％,and respectively decreased soil C∶P and N∶P,plant C∶P and N∶P,and soil microbial biomass N∶P by 29.5％to 51.5％,30.1％to 54.3％,13.2％to 28.3％,6.71％to 19.8％,and 29.1％to 52.7％.(5)With the extension of planting years,by 2016,soil C,N,C∶N,plant N:P,and soil microbial bio-mass C∶P had cumulatively increased by 49.3％、33.9％、11.3％、77.6％,and 126.0％,respectively compared to 2011,while plant phosphorus content had cumulatively decreased by 51.3％.In summary,the growth of alfalfa and soil microorganisms was limited by N deficiency under mulching with ridge and furrow and a high amount of P fertilizer.This management practice accelerated the decomposition of soil organic matter,which was not conducive to maintaining the growth of alfalfa and soil fertility in the semi-arid region of the Loess Plateau.Therefore,when the amount of P applied under mulching with ridge and furrow is higher than 22.7 kg·hm-2,appropriate nitrogen fertilizer should be supplied.关键词
垄沟覆膜/施磷/紫花苜蓿/土壤-植物-微生物系统/生态化学计量Key words
ridge and furrow mulching/phosphorus application/Medicago sativa L./soil-plant-microorganism system/ecological stoichiometry分类
农业科技引用本文复制引用
徐秋韵,周伟迪,韩成龙,谷艳杰,李凤民..垄沟覆膜和施磷对紫花苜蓿地土壤-植物-微生物系统生态化学计量的影响[J].干旱地区农业研究,2025,43(5):148-158,11.基金项目
国家自然科学基金(31960625) （31960625）
青海省科技计划自然科学基金青年项目(2020-ZJ-969Q) （2020-ZJ-969Q）
