高师理科学刊2025，Vol.45Issue(10)：33-39,7.DOI:10.3969/j.issn.1007-9831.2025.10.006
曼地亚红豆杉根际促生菌的分离及促生性能
Isolation and growth-promoting properties of plant growth promoting rhizobacteria from Taxus×media rhizosphere
摘要
Abstract
Took the rhizosphere soil of Taxus×media as the research object and isolated and identified plant growth-promoting rhizobacteria(PGPR)that can promote its growth.Strains with growth-promoting effects were screened through growth-promoting experiments such as phosphorus solubilization,potassium solubilization,siderophore production,indole acetic acid(IAA)production,based on the wheat germination experiments and Taxus×media cutting experiments,identified specific PGPR strains with significant growth-enhancing effects on Taxus×media.The results showed that,among the 13 strains with different morphologies screened from the rhizosphere soil of Taxus×media,the WC strain possesses the ability to solubilize potassium;the W1,W7,W8,WA,WB,WC,WD and WE strains could produce siderophores;the W3,W7,W8 and WB strains could produce IAA.The results of the wheat germination experiment showed that the screened growth-promoting bacteria had significant growth-promoting effects on the plant height of wheat.The growth-promoting effects of the W1,W8,WA and WB strains were more obvious,but only the WC strain had a good promoting effect on root length.The results of the Taxus×media cutting experiment showed that the W1,WA,WC,WD strains the mixed bacteria of WB and WC had a significant promoting effect on the growth of new branches,the mixed bacteria of WB and WC had a significant promoting effect on the growth of bud scales.Based on the above experimental results,the 16S rDNA of the W1,W8,WA,WB,WC and WD strains were sequenced,which were Exiguobacterium acetylicum,Bacillus mobilis,Bacillus albus,Lysinibacillus sphaericus,Lysinibacillus fusiformis and Escherichia coli respectively.The study provides reliable microbial resources for the research and development of Taxus×media microbial fertilizers and reference basis for Taxus×media cultivation.关键词
曼地亚红豆杉/根际促生菌/小麦萌发/曼地亚红豆杉扦插/16S rDNA测序Key words
Taxus×media/rhizosphere growth-promoting bacteria/wheat germination/Taxus×media cuttings/16S rDNA sequencing分类
生物学引用本文复制引用
王淼,程子烨,郑莹鑫,刁毅柔,程亚蕊..曼地亚红豆杉根际促生菌的分离及促生性能[J].高师理科学刊,2025,45(10):33-39,7.基金项目
国家自然科学基金项目(32400051) （32400051）
汉江师范学院重点项目(XJ2022A02) （XJ2022A02）
