寒旱农业科学2025，Vol.4Issue(10)：963-968,6.DOI:10.3969/j.issn.2097-2172.2025.10.014
河西灌区梨火疫病菌鉴定及田间药剂筛选
Pathogen Identification of Pear Fire Blight and Field Screening of Chemical Treatments in the Hexi Irrigation Region
张文波 1何树文 1杨成德 2郭致杰 3郑婷燕 1许永锋1
作者信息
- 1. 张掖市植保植检站,甘肃 张掖 734000
- 2. 甘肃农业大学植物保护学院,甘肃 兰州 730070
- 3. 甘肃省农业科学院植物保护研究所,甘肃 兰州 730070
- 折叠
摘要
Abstract
This experiment focused on isolating,identifying pathogens causing pear fire blight in the Hexi Irrigation Region and screening effective chemical treatments for field application,so as to provide a scientific basis for diagnosis and management strategies of Zaosu pears.The pathogen was isolated and identified using the plate streak method,followed by morphological characterization and genetic sequence analysis.Pathogenicity testing confirmed that isolate F2 was the causal agent of pear fire blight.The bacterium was Gram-negative,with hemispherical,convex,milky-white,and viscous colonies with smooth and moist surfaces and neat edges.After 3 days of incubation at 28℃,colony diameters ranged from 3 to 4 mm.Bacteria somatic was rod-shaped,measuring(0.9 to 1.8)μm×(0.6 to 1.5)μm,occurring mostly singly,occasionally in pairs or short chains of 3 to 4 bacterial cells.Based on morphological,cultural,and molecular characteristics,the pathogen was identified as Erwinia amylovora.Field efficacy trials revealed that 3%thiamphenicol water dispersible granules at 1 000×dilution exhibited the highest control efficacy of 61.9%at 15 days post-application,whereas 4%kasugamy cinaqueous solution at 1 200×dilution demonstrated demonstrated superior efficacy of 62.4%at 30 days post-application.All other treatments maintained control efficiencies above 50%.Therefore,both thiamphenicol water dispersible granules at 1 000×dilution and 4%kasugamycin aqueous solution at 1 200×dilution are recommended for field application in pear fire blight management.关键词
梨火疫病/病原菌鉴定/田间防效/药剂筛选/河西灌区Key words
Pear fire blight/Pathogen identification/Field efficacy/Chemical screenirg/Hexi Irrigation Region分类
园艺学与植物营养学引用本文复制引用
张文波,何树文,杨成德,郭致杰,郑婷燕,许永锋..河西灌区梨火疫病菌鉴定及田间药剂筛选[J].寒旱农业科学,2025,4(10):963-968,6.
