光学精密工程2025，Vol.33Issue(19)：3021-3031,11.DOI:10.37188/OPE.20253319.3021
运用频闪照明的弹光调制型双折射显微成像
Birefringence microscopy imaging based on photoelastic modulation with stroboscopic illumination
摘要
Abstract
To achieve rapid and precise birefringence microscopy,a photoelastic-modulation-based imag-ing scheme is proposed.Leveraging the high modulation frequency and purity of photoelastic modulators,birefringence imaging measurements are realized.The disparity between the modulator's high-frequency operation and the camera's low frame rate is resolved via stroboscopic illumination.The sample's birefrin-gence phase retardation distribution is retrieved from only three images acquired at photoelastic modulation phases of 0°,30°,and 90°.An experimental system was constructed and tested on a wave plate and linden stem sections.Results indicate a wave-plate retardation error below 1%,retardation accuracy within±λ/300,and a maximum retardation-distribution deviation not exceeding 1.62 nm.Distinct retardation regions in the linden stem sections enabled differentiation of anatomical parts,with single-measurement ac-quisition time under 3 ms.The method achieves high-speed,high-precision mapping of birefringence phase retardation and offers an advanced technical approach for studies of two-dimensional materials,bio-logical tissue imaging,and related fields.关键词
显微成像/弹光调制/频闪照明/相位延迟量/双折射Key words
microscopy imaging/polarization modulation/stroboscopic illumination/phase retardation/birefringence分类
物理学引用本文复制引用
董业,任书宣,王志斌,王爽,李克武..运用频闪照明的弹光调制型双折射显微成像[J].光学精密工程,2025,33(19):3021-3031,11.基金项目
国家自然科学基金资助项目(No.63305309,No.62205310) （No.63305309,No.62205310）
