海南医科大学学报2025，Vol.31Issue(20)：1556-1564,9.DOI:10.13210/j.cnki.jhmu.20250623.001
三七总皂苷改善抑郁样行为小鼠生精功能实验研究
Experimental study of Panax notoginseng saponins on improving spermatogenic function in mice with depression-like behavior
摘要
Abstract
Objective:To explore the effect of Panax notoginseng saponin(PNS)on spermatogenesis in mice with depression-like behavior and its possible mechanism.Methods:A total of 40 male C57BL/6J mice were randomly divided into the Control group and the model group(Complete Freund's Adjuvant,CFA).The depression-like behavior model was induced by injecting CFA into the sole of the right hind paw of mice in the model group.After the establishment of depression-like behavior model,the mice were divided into the model group(CFA-D group)and the drug intervention group(CFA-D+PNS group).Open field ex-periment was used to detect the activity of mice,sugar spray experiment and forced swimming experiment were used to detect depression-like behavior of mice.Testes and epididymis weight,testes coefficient,epididymis coefficient,and sperm count were detected to check reproductive organs and sperm count of mice.Morphological changes of testicular spermatogenic cells and sperm deformities were observed by H&E staining.The changes of serum testosterone(T),luteinizing hormone(LH),gonadotropin re-leasing hormone(GnRH),and follicle stimulating hormone(FSH)were detected by ELISA assay.Results:Compared to the Control group,the activity time(P<0.001)and activity distance(P<0.001)in the model group were significantly reduced,the back licking time was reduced(P<0.001),and the immobile time of forced swimming was prolonged(P<0.01).Testicular weight(P<0.001),epididymal weight(P<0.01),testicular coefficient(P<0.001),epididymal coefficient(P<0.001)and sperm count(P<0.001)were all decreased,and the malform rates of sperm head(P<0.001),neck(P<0.05)and tail(P<0.001)were significantly increased.The number of spermatogenic epithelial cells decreased and the expression of T(P<0.01),FSH(P<0.001),GnRH(P<0.001),LH(P<0.001)in serum decreased significantly.After PNS intervention,the inner frame activity time(P<0.01)and activity distance(P<0.001)were significantly increased,the back licking time was extended(P<0.001),and the immobile time of forced swimming was shorter(P<0.01).Testicular weight(P<0.001),epididymal weight(P<0.01),testicular coefficient(P<0.001),epididymal coefficient(P<0.05),and sperm count(P<0.01)were signifi-cantly increased,and the malform rates of sperm head(P<0.01),neck(P<0.05),and tail(P<0.01)were significantly de-creased.The spermatogenic epithelial cells were closely arranged and increased in number.Meanwhile,serum T(P<0.05),FSH(P<0.001),GnRH(P<0.01),LH(P<0.001)were increased.Conclusion:PNS can significantly improve reproductive organ atrophy and spermatogenic dysfunction in depression-like mice.At the same time,the concentrations of T,LH,GnRH,and FSH in serum were increased,thus improving spermatogenic function of depression-like behavior mice.关键词
三七总皂苷/抑郁样行为/生精功能Key words
Panax notoginseng saponin/Depression-like behavior/Spermatogenic function分类
中医学引用本文复制引用
万力,杨智伟,马瑞,郑瑶,覃欧翔宇,高静,何洁,刘国文..三七总皂苷改善抑郁样行为小鼠生精功能实验研究[J].海南医科大学学报,2025,31(20):1556-1564,9.基金项目
This study was supported by the Natural Science Foundation Project of Hainan Province(824MS065) （824MS065）
Key Laboratory of Longevity and Age-related Diseases of the Ministry of Education Funded Project(KLLAD202305)海南省自然科学基金资助项目(824MS065) （KLLAD202305）
长寿与老年相关疾病教育部重点实验室资助项目(KLLAD202305) （KLLAD202305）
