海南医科大学学报2025，Vol.31Issue(20)：1573-1579,1590,8.DOI:10.13210/j.cnki.jhmu.20250115.004
益肺通络方对肺纤维化模型大鼠IL-17RA/TRAF6/ACT1信号通路及肝肾功能影响的研究
Study on the effects of Yifei Tongluo Formula on IL-17RA/TRAF6/ACT1 signaling pathway and liver and kidney function in pulmonary fibrosis model rats
摘要
Abstract
Objective:To investigate the effects of Yifei Tongluo Formula on the IL-17RA/TRAF6/ACT1 signaling path-way and liver and kidney function in an animal model of pulmonary fibrosis established by using bleomycin tracheal instillation method.Methods:The SD rat with pulmonary fibrosis was constructed using bleomycin tracheal instillation method,and random-ly divided into 5 groups:the blank control group,the model group,the prednisone group,the low-dose Yifei Tongluo Formula group,and the high-dose Yifei Tongluo Formula group.Corresponding interventions were given for 14 days,and rat serum and lung tissue were taken for testing.The relative expression of IL-17RA,TRAF6,ACT1,IL-1β,TNF-α,and IL-6 mRNA was detected by polymerase chain reaction(PCR),and the protein expression of IL-17RA,TRAF6,and ACT1 was detected by Western blot(WB).The expression of signal molecules TRAF6 and ACT1 was detected by immunofluorescence.The levels of serum cytokines IL-1β and TNF-α in rats were assessed using an enzyme-linked immunosorbent assay(ELISA),and liver and kidney function indicators were detected by a fully automated biochemical analyzer.Results:The immunofluorescence images indi-cated that the fluorescence intensity of the signal molecules TRAF6 and ACT1 in the lung tissue of the model group rats was ele-vated when compared to the blank control group.Furthermore,a significant decrease in fluorescence intensity was observed follow-ing the intervention with Yifei Tongluo Formula.The results from the PCR analysis indicated that,in comparison to the blank con-trol group,the model group rats exhibited a notable increase in the relative expression levels of IL-17RA,TRAF6,ACT1,IL-1β,TNF-α,and IL-6 mRNA(P<0.05).Additionally,when compared to the model group,the Yifei Tongluo Formula group rats demonstrated a significant decrease in the relative expression of the aforementioned genes(P<0.05).The results of the WB test indicated that the expression levels of IL-17RA,TRAF6,and ACT1 proteins in the lung tissues of the rats in the model group were significantly higher compared to those in the blank control group(P<0.05).Additionally,when compared to the mod-el group,the Yifei Tongluo Formula demonstrated a significant inhibitory effect on the expression of these proteins(P<0.05).The results from the ELISA indicated that the model group rats exhibited a marked increase in the cytokine levels of IL-1β and TNF-α compared to the blank control group(P<0.05).Furthermore,when compared to the model group,there was a significant reduction in the levels of these cytokines in the group of rats treated with the Yifei Tongluo Formula(P<0.05).The results of the fully automated biochemical analyzer showed that the liver and kidney function-related indicators of rats were within the normal range,and there was no statistical difference between the groups(P>0.05).Conclusion:Yifei Tongluo Formula may exert anti pulmonary fibrosis effects by regulating the IL-17RA/TRAF6/ACT1 signaling pathway.Yifei Tongluo Formula exerts anti-inflammatory effects by downregulating the secretion of IL-17A related cytokines and has no toxic side effects on liver and kid-ney function.关键词
肺纤维化/益肺通络方/IL-17A/实验研究Key words
Idiopathic pulmonary fibrosis/Yifei Tongluo Formula/IL-17A/Experimental study分类
中医学引用本文复制引用
马先,冯家腾,周博文,李泽峰,刘骅漫,徐飞,辛玲杰,贾新华..益肺通络方对肺纤维化模型大鼠IL-17RA/TRAF6/ACT1信号通路及肝肾功能影响的研究[J].海南医科大学学报,2025,31(20):1573-1579,1590,8.基金项目
This study was supported by the 2022 Young Qihuang Scholar Training Program[Guo Zhong Yao Ren Jiao Han(2022)No.256] （2022）
Shandong Province High-level Talent Cultivation Project for Traditional Chinese Medicine[Lu Wei Han(2023)No.143]2022年青年岐黄学者培养项目[国中医药人教函(2022)256号] （2023）
山东省中医药高层次人才培育项目[鲁卫函(2023)143号] （2023）
