海南医科大学学报2025，Vol.31Issue(20)：1580-1590,11.DOI:10.13210/j.cnki.jhmu.20241225.001
基于网络药理学与分子对接技术探讨EGCG缓解1型糖尿病认知功能障碍的作用研究
Role of EGCG in alleviating cognitive dysfunction in type 1 diabetes mellitus based on network pharmacology and molecular docking technology
摘要
Abstract
Objective:To provide insight into the role of epigallocatechin gallate and its underlying mechanisms in the treat-ment of cognitive dysfunction associated with type 1 diabetes mellitus(T1DM)by means of experimental validation and network pharmacology.Methods:SD rats were randomly grouped into a normal control group and a T1DM model group,and the T1DM model was constructed via a one-time intraperitoneal injection of 65 mg/kg streptozotocin(STZ).After the successful establish-ment of T1DM diabetic rat model,it was further divided into three subgroups:untreated T1DM model group(T1DM group),the EGCG-treated group(T1DM+EGCG group)and nerve growth factor(NGF)-treated T1DM group(T1DM+NGF group).Meanwhile,the normal control group was further divided into two groups,the untreated control(Control)group and the EGCG-treated group alone(EGCG group).The drug was administered continuously for 8 weeks,during which the blood glucose levels of rats in each group were monitored and their spatial learning and memory abilities were tested using Morris water maze.Further,the potential targets of EGCG action were screened by resources such as TCMSP database,UniProt database and Gene-Cards,while the protein-protein interaction(PPI)network was established by the integrated use of String database;Metascape was used for the enrichment analysis of GO function and KEGG pathway;and the molecular docking experiments were performed with the help of AutoDock software to predict the binding affinity of EGCG to key targets.Results:It was demonstrated that EGCG significantly improved the cognitive dysfunction caused by T1DM.Network pharmacological studies revealed that AKT1,P53,TNF and IL-6 were the core targets of EGCG in alleviating T1DM-related cognitive dysfunction.Molecular docking experi-ments showed that EGCG could effectively bind to the above targets.Conclusion:EGCG is effective in improving cognitive dys-function in patients with T1DM,and its mechanism of action may be related to its effects on multiple key targets and signalling pathways as well as the reduction of blood glucose levels.关键词
表没食子儿茶素没食子酸酯/1型糖尿病/认知功能障碍/网络药理学/关键靶点/分子对接Key words
Epigallocatechin gallate/Type 1 diabetes/Cognitive dysfunction/Network pharmacology/Key targets/Molec-ular docking分类
中医学引用本文复制引用
杨思垚,李涵飞,乔静晓,陈宇静,祝田田..基于网络药理学与分子对接技术探讨EGCG缓解1型糖尿病认知功能障碍的作用研究[J].海南医科大学学报,2025,31(20):1580-1590,11.基金项目
This study was supported by the Key Research Project of Higher Education Institutions in Henan Province(22A180010)河南省高等学校重点科研项目(22A180010) （22A180010）
