华北农学报2025，Vol.40Issue(5)：1-11,11.DOI:10.7668/hbnxb.20195902
小麦耐低温关键调控途径解析及TaGGCT18-6A基因功能验证
Dissection of Key Regulatory Pathways for Low-temperature Tolerance and Functional Verification of TaGGCT18-6A Gene in Wheat
摘要
Abstract
To elucidate the mechanisms of cold response and explore superior cold-tolerance gene resources in wheat,this study employed transcriptome sequencing to uncover key regulatory networks underlying wheat cold response,and performed functional validation of the candidate gene TaGGCT18-6A.The results demonstrated that 4 ℃ cold treat-ments induced 10 893 and 18 784 differentially expressed genes(DEGs)in wheat seedlings after 6 h and 24 h cold treat-ment,respectively.KEGG analysis revealed significant enrichment of DEGs in pathways including MAPK signaling trans-duction and glutathione metabolism.A γ-glutamyl cyclotransferase gene TaGGCT18-6A was cloned through screening key genes in glutathione metabolism.This gene had a length of 1 772 bp,encoding 218 amino acids with conserved GGCT-like superfamily and ChaC core domain.Promoter cis-acting element analysis identified stress-responsive elements such as low-temperature-responsive(LTR)and dehydration-responsive(DRE)elements;consistently,expression pattern analysis showed sustained upregulation of TaGGCT18-6A under 4℃cold treatments.Functional validation in transgenic rice re-vealed that overexpression lines OE#1,OE#2 and OE#3 exhibited significantly enhanced survival rate,plant height,and biomass.Overexpression lines OE#1 and OE#2 exhibited significantly enhanced plant height,while overexpression lines OE#1,OE#2 and OE#3 exhibited significantly reduced relative electrolyte leakage under-4 ℃ freezing treatments.After 4 ℃ treatment,overexpression lines accumulated higher levels of osmolytes(proline and soluble sugars),decreased ma-londialdehyde(MDA)content,and increased activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT).These findings collectively demonstrated that TaGGCT18-6A enhanced plant cold tolerance by regulating glutathi-one metabolism to improve antioxidant capacity.This study will provide a theoretical foundation and valuable genetic re-sources for molecular breeding of cold-tolerant wheat.关键词
小麦/低温胁迫/转录组分析/γ-谷氨酰环转移酶/TaGGCT18-6AKey words
Wheat/Low temperature stress/Transcriptome analysis/γ-glutamyl cyclotransferase/TaGGCT18-6A分类
生物学引用本文复制引用
成春华,陈涛,张龙,郭利建,车卓,马靖福,杨德龙..小麦耐低温关键调控途径解析及TaGGCT18-6A基因功能验证[J].华北农学报,2025,40(5):1-11,11.基金项目
甘肃省农业农村厅种业攻关项目(GYGG-2024-2) （GYGG-2024-2）
甘肃省科技重大专项(22ZD6NA009) （22ZD6NA009）
甘肃省科技厅基础研究创新群体项目(24JRRA633) （24JRRA633）
中央引导地方科技发展资金项目(23ZYQA0322) （23ZYQA0322）
甘肃省高校科研创新平台重点培育项目(2024CXPT-01) （2024CXPT-01）
甘肃省高等学校产业支撑计划项目(2022CYZC-4) （2022CYZC-4）
甘肃省重点研发计划项目(25YFWA020) （25YFWA020）
甘肃农业大学博士科研启动项目(GAU-KYQD-2020-6) （GAU-KYQD-2020-6）
