华北农学报2025，Vol.40Issue(5)：12-19,8.DOI:10.7668/hbnxb.20195906
小麦TaWRKY70的克隆、亚细胞定位与表达分析
Cloning,Subcellular Localization and Expression Analysis of TaWRKY70 in Wheat
摘要
Abstract
To elucidate the role of WRKY transcription factor family members in the dynamic regulation of wheat growth and development,as well as in responses to abiotic stresses,this study investigated the expression patterns of Ta WRKY gene under drought,high salinity,and low-temperature stress conditions.Using the common wheat cultivar Chinese Spring as the experimental material,we obtained the TaWRKY70 gene through molecular cloning.The coding sequence of TaWRKY70 was 885 bp in length,encoding a 294-amino-acid hydrophilic and unstable protein.Bioinfor-matics analysis revealed that the protein possessed a typical WRKYGQK conserved domain and a C2HC-type zinc fin-ger structure,classifying it as a Group Ⅲ WRKY transcription factor.Cis-regulatory element analysis of the TaWRKY70 promoter region identified regulatory elements involved in responses to methyl jasmonate,abscisic acid,and ethylene.Co-expression gene analysis suggested that TaWRKY70 was associated with multiple stress response processes in wheat,including hormone signaling,defense against microbial pathogens,and responses to cold stress.Phylogenetic analysis indicated that TaWRKY70 shared a close evolutionary relationship with WRKY70 proteins from other Poaceae species,such as barley,maize,sorghum,and foxtail millet.Subcellular localization experiment fur-ther confirmed that TaWRKY70 was localized in the nucleus,consistent with the characteristics of a transcription fac-tor.Expression pattern analysis showed that TaWRKY70 was expressed in wheat roots,stems,leaves,young spikes,and grains,with higher expression levels observed in roots and leaves.Under abiotic stress conditions,TaWRKY70 expression was downregulated in response to abscisic acid and low-temperature treatments but upregulated under salicylic acid,NaCl,PEG6000,and high-temperature treatments.In conclusion,the cloning of TaWRKY70 gene and analysis of its expression pattern provide a basis for the next step to analyze the molecular mechanism of TaWRKY70 involved in wheat stress resistance.关键词
小麦/TaWRKY70/基因克隆/亚细胞定位/表达分析Key words
Wheat/TaWRKY70/Gene cloning/Subcellular localization/Expression analysis分类
农业科技引用本文复制引用
卢振华,王俊斌,梁晨,张丽,佟可心,陈小强,李明,丁博,邱丽娜,谢晓东..小麦TaWRKY70的克隆、亚细胞定位与表达分析[J].华北农学报,2025,40(5):12-19,8.基金项目
国家自然科学基金项目(32301817) （32301817）
天津市自然科学基金项目(22JCQNJC01470) （22JCQNJC01470）
河北省教育厅科学研究项目(YJZ2024001) （YJZ2024001）
